In vivo SERS monitoring in plants using plasmonic nanoprobes

Conference Paper

Plant biotechnology and biofuel research is critical in addressing increasing global demands for energy. Further understanding of biomass producing associated metabolic pathways in plants can be used to exploit and increase the production of biomass for energy purposes. In vivo detection of biomarkers associated with plant growth for bioenergy has proved to be limited due to complex sample preparation required by traditional methods. In addition, genetic transformation and biomolecule monitoring inside plant cells is regulated by diameter and size exclusion limits of the plant cell wall (5 - 20 nm). Currently limited methods exist for enabling direct entry into plant cells. Moreover, these methods, such as biolistic particle delivery and electroporation use mechanical force that causes damages to the plant tissue. Nanoparticles could serve as promising platforms for probes to characterize intercellular and intracellular plant biomarkers and pathways. Bi-metallic nanostars are a plasmonics-active nanoplatform capable of high surface-enhanced Raman scattering (SERS) which can enter plant cells and have the future potential for nucleic acid sensing. Imaging technologies such as SERS mapping, confocal imaging, X-ray fluorescence imaging, multi-photon imaging, and transmission electron microscopy have been utilized to determine the compartmentalization and location of the SERS iMS biosensors inside Arabidopsis plants.

Full Text

Duke Authors

Cited Authors

  • Cupil-Garcia, V; Li, JQ; Odion, R; Strobbia, P; Crawford, BM; Wang, HN; Hu, J; Zentella, R; Kemner, KM; Sun, TP; Vo-Dinh, T

Published Date

  • January 1, 2022

Published In

Volume / Issue

  • 11978 /

International Standard Serial Number (ISSN)

  • 1605-7422

International Standard Book Number 13 (ISBN-13)

  • 9781510648272

Digital Object Identifier (DOI)

  • 10.1117/12.2617364

Citation Source

  • Scopus