Skip to main content
Journal cover image

Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity.

Publication ,  Journal Article
Dong, C; Chua, A; Ganguly, B; Krensky, AM; Clayberger, C
Published in: J Immunol Methods
July 2005

Chemokines are a family of small, secreted chemoattractant cytokines that regulate distribution and function of leukocytes during immune responses. While most chemokines are members of the CC or CXC subgroups, XCL1, also known as lymphotactin, is the sole member of the C subgroup. XCL1 is produced by activated CD8(+) T cells, NK cells, gammadelta T cells, and mast cells. XCL1 differs from other chemokines in that it contains only a single disulfide bond and a mucin-like domain at its carboxy terminus that is glycosylated. Understanding the biologic functions of chemokines has largely depended upon expression of these recombinant molecules in E. coli. To examine the effects of glycosylation on the biologic activity of XCL1, we designed constructs for expression of human XCL1 in insect S2 cells. Comparison of this material with that expressed in E. coli reveals that glycosylation significantly increases the biologic activity of XCL1.

Duke Scholars

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

July 2005

Volume

302

Issue

1-2

Start / End Page

136 / 144

Location

Netherlands

Related Subject Headings

  • Transfection
  • Recombinant Proteins
  • Immunology
  • Humans
  • Glycosylation
  • Escherichia coli
  • Drosophila melanogaster
  • Chemotaxis, Leukocyte
  • Chemokines, C
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Dong, C., Chua, A., Ganguly, B., Krensky, A. M., & Clayberger, C. (2005). Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. J Immunol Methods, 302(1–2), 136–144. https://doi.org/10.1016/j.jim.2005.05.008
Dong, Chen, Annabelle Chua, Bishu Ganguly, Alan M. Krensky, and Carol Clayberger. “Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity.J Immunol Methods 302, no. 1–2 (July 2005): 136–44. https://doi.org/10.1016/j.jim.2005.05.008.
Dong C, Chua A, Ganguly B, Krensky AM, Clayberger C. Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. J Immunol Methods. 2005 Jul;302(1–2):136–44.
Dong, Chen, et al. “Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity.J Immunol Methods, vol. 302, no. 1–2, July 2005, pp. 136–44. Pubmed, doi:10.1016/j.jim.2005.05.008.
Dong C, Chua A, Ganguly B, Krensky AM, Clayberger C. Glycosylated recombinant human XCL1/lymphotactin exhibits enhanced biologic activity. J Immunol Methods. 2005 Jul;302(1–2):136–144.
Journal cover image

Published In

J Immunol Methods

DOI

ISSN

0022-1759

Publication Date

July 2005

Volume

302

Issue

1-2

Start / End Page

136 / 144

Location

Netherlands

Related Subject Headings

  • Transfection
  • Recombinant Proteins
  • Immunology
  • Humans
  • Glycosylation
  • Escherichia coli
  • Drosophila melanogaster
  • Chemotaxis, Leukocyte
  • Chemokines, C
  • Cells, Cultured