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Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike.

Publication ,  Journal Article
Stalls, V; Lindenberger, J; Gobeil, SM-C; Henderson, R; Parks, R; Barr, M; Deyton, M; Martin, M; Janowska, K; Huang, X; May, A; Speakman, M ...
Published in: Cell Rep
June 28, 2022

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron BA.2 sub-lineage has gained in proportion relative to BA.1. Because spike (S) protein variations may underlie differences in their pathobiology, here we determine cryoelectron microscopy (cryo-EM) structures of the BA.2 S ectodomain and compare these with previously determined BA.1 S structures. BA.2 receptor-binding domain (RBD) mutations induce remodeling of the RBD structure, resulting in tighter packing and improved thermostability. Interprotomer RBD interactions are enhanced in the closed (or 3-RBD-down) BA.2 S, while the fusion peptide is less accessible to antibodies than in BA.1. Binding and pseudovirus neutralization assays reveal extensive immune evasion while defining epitopes of two outer RBD face-binding antibodies, DH1044 and DH1193, that neutralize both BA.1 and BA.2. Taken together, our results indicate that stabilization of the closed state through interprotomer RBD-RBD packing is a hallmark of the Omicron variant and show differences in key functional regions in the BA.1 and BA.2 S proteins.

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Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

June 28, 2022

Volume

39

Issue

13

Start / End Page

111009

Location

United States

Related Subject Headings

  • Spike Glycoprotein, Coronavirus
  • SARS-CoV-2
  • Receptors, Virus
  • Humans
  • Cryoelectron Microscopy
  • COVID-19
  • Antibodies, Viral
  • 31 Biological sciences
  • 1116 Medical Physiology
  • 0601 Biochemistry and Cell Biology
 

Citation

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Stalls, V., Lindenberger, J., Gobeil, S.-C., Henderson, R., Parks, R., Barr, M., … Acharya, P. (2022). Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike. Cell Rep, 39(13), 111009. https://doi.org/10.1016/j.celrep.2022.111009
Stalls, Victoria, Jared Lindenberger, Sophie M-C Gobeil, Rory Henderson, Rob Parks, Maggie Barr, Margaret Deyton, et al. “Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike.Cell Rep 39, no. 13 (June 28, 2022): 111009. https://doi.org/10.1016/j.celrep.2022.111009.
Stalls V, Lindenberger J, Gobeil SM-C, Henderson R, Parks R, Barr M, et al. Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike. Cell Rep. 2022 Jun 28;39(13):111009.
Stalls, Victoria, et al. “Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike.Cell Rep, vol. 39, no. 13, June 2022, p. 111009. Pubmed, doi:10.1016/j.celrep.2022.111009.
Stalls V, Lindenberger J, Gobeil SM-C, Henderson R, Parks R, Barr M, Deyton M, Martin M, Janowska K, Huang X, May A, Speakman M, Beaudoin E, Kraft B, Lu X, Edwards RJ, Eaton A, Montefiori DC, Williams WB, Saunders KO, Wiehe K, Haynes BF, Acharya P. Cryo-EM structures of SARS-CoV-2 Omicron BA.2 spike. Cell Rep. 2022 Jun 28;39(13):111009.
Journal cover image

Published In

Cell Rep

DOI

EISSN

2211-1247

Publication Date

June 28, 2022

Volume

39

Issue

13

Start / End Page

111009

Location

United States

Related Subject Headings

  • Spike Glycoprotein, Coronavirus
  • SARS-CoV-2
  • Receptors, Virus
  • Humans
  • Cryoelectron Microscopy
  • COVID-19
  • Antibodies, Viral
  • 31 Biological sciences
  • 1116 Medical Physiology
  • 0601 Biochemistry and Cell Biology