Warming Induces Significant Reprogramming of Beige, but Not Brown, Adipocyte Cellular Identity.

Journal Article (Journal Article)

Beige and brown adipocytes generate heat in response to reductions in ambient temperature. When warmed, both beige and brown adipocytes exhibit morphological "whitening," but it is unknown whether or to what extent this represents a true shift in cellular identity. Using cell-type-specific profiling in vivo, we uncover a unique paradigm of temperature-dependent epigenomic plasticity of beige, but not brown, adipocytes, with conversion from a brown to a white chromatin state. Despite this profound shift in cellular identity, warm whitened beige adipocytes retain an epigenomic memory of prior cold exposure defined by an array of poised enhancers that prime thermogenic genes for rapid response during a second bout of cold exposure. We further show that a transcriptional cascade involving glucocorticoid receptor and Zfp423 can drive warm-induced whitening of beige adipocytes. These studies identify the epigenomic and transcriptional bases of an extraordinary example of cellular plasticity in response to environmental signals.

Full Text

Duke Authors

Cited Authors

  • Roh, HC; Tsai, LTY; Shao, M; Tenen, D; Shen, Y; Kumari, M; Lyubetskaya, A; Jacobs, C; Dawes, B; Gupta, RK; Rosen, ED

Published Date

  • May 1, 2018

Published In

Volume / Issue

  • 27 / 5

Start / End Page

  • 1121 - 1137.e5

PubMed ID

  • 29657031

Pubmed Central ID

  • PMC5932137

Electronic International Standard Serial Number (EISSN)

  • 1932-7420

Digital Object Identifier (DOI)

  • 10.1016/j.cmet.2018.03.005

Language

  • eng

Conference Location

  • United States