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Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice.

Publication ,  Journal Article
Vishvanath, L; MacPherson, KA; Hepler, C; Wang, QA; Shao, M; Spurgin, SB; Wang, MY; Kusminski, CM; Morley, TS; Gupta, RK
Published in: Cell Metab
February 9, 2016

The expansion of white adipose tissue (WAT) in obesity involves de novo differentiation of new adipocytes; however, the cellular origin of these cells remains unclear. Here, we utilize Zfp423(GFP) reporter mice to characterize adipose mural (Pdgfrβ(+)) cells with varying levels of the preadipocyte commitment factor Zfp423. We find that adipose tissue contains distinct mural populations, with levels of Zfp423 distinguishing adipogenic from inflammatory-like mural cells. Using our "MuralChaser" lineage tracking system, we uncover adipose perivascular cells as developmental precursors of adipocytes formed in obesity, with adipogenesis and precursor abundance regulated in a depot-dependent manner. Interestingly, Pdgfrβ(+) cells do not significantly contribute to the initial cold-induced recruitment of beige adipocytes in WAT; it is only after prolonged cold exposure that these cells differentiate into beige adipocytes. These results provide genetic evidence for a mural cell origin of white adipocytes in obesity and suggest that beige adipogenesis may originate from multiple sources.

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Published In

Cell Metab

DOI

EISSN

1932-7420

Publication Date

February 9, 2016

Volume

23

Issue

2

Start / End Page

350 / 359

Location

United States

Related Subject Headings

  • Transcription Factors
  • Stem Cells
  • Receptor, Platelet-Derived Growth Factor beta
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Male
  • Inflammation
  • Hyperplasia
  • Female
  • Feeding Behavior
 

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Vishvanath, L., MacPherson, K. A., Hepler, C., Wang, Q. A., Shao, M., Spurgin, S. B., … Gupta, R. K. (2016). Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice. Cell Metab, 23(2), 350–359. https://doi.org/10.1016/j.cmet.2015.10.018
Vishvanath, Lavanya, Karen A. MacPherson, Chelsea Hepler, Qiong A. Wang, Mengle Shao, Stephen B. Spurgin, Margaret Y. Wang, Christine M. Kusminski, Thomas S. Morley, and Rana K. Gupta. “Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice.Cell Metab 23, no. 2 (February 9, 2016): 350–59. https://doi.org/10.1016/j.cmet.2015.10.018.
Vishvanath L, MacPherson KA, Hepler C, Wang QA, Shao M, Spurgin SB, et al. Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice. Cell Metab. 2016 Feb 9;23(2):350–9.
Vishvanath, Lavanya, et al. “Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice.Cell Metab, vol. 23, no. 2, Feb. 2016, pp. 350–59. Pubmed, doi:10.1016/j.cmet.2015.10.018.
Vishvanath L, MacPherson KA, Hepler C, Wang QA, Shao M, Spurgin SB, Wang MY, Kusminski CM, Morley TS, Gupta RK. Pdgfrβ+ Mural Preadipocytes Contribute to Adipocyte Hyperplasia Induced by High-Fat-Diet Feeding and Prolonged Cold Exposure in Adult Mice. Cell Metab. 2016 Feb 9;23(2):350–359.
Journal cover image

Published In

Cell Metab

DOI

EISSN

1932-7420

Publication Date

February 9, 2016

Volume

23

Issue

2

Start / End Page

350 / 359

Location

United States

Related Subject Headings

  • Transcription Factors
  • Stem Cells
  • Receptor, Platelet-Derived Growth Factor beta
  • Mice, Transgenic
  • Mice, Inbred C57BL
  • Male
  • Inflammation
  • Hyperplasia
  • Female
  • Feeding Behavior