Distinct regulatory mechanisms governing embryonic versus adult adipocyte maturation.

Journal Article (Journal Article)

Pathological expansion of adipose tissue contributes to the metabolic syndrome. Distinct depots develop at various times under different physiological conditions. The transcriptional cascade mediating adipogenesis is established in vitro, and centres around a core program involving PPARγ and C/EBPα. We developed an inducible, adipocyte-specific knockout system to probe the requirement of key adipogenic transcription factors at various stages of adipogenesis in vivo. C/EBPα is essential for all white adipogenic conditions in the adult stage, such as adipose tissue regeneration, adipogenesis in muscle and unhealthy expansion of white adipose tissue during high-fat feeding or due to leptin deficiency. Surprisingly, terminal embryonic adipogenesis is fully C/EBPα independent, but does however depend on PPARγ; cold-induced beige adipogenesis is also C/EBPα independent. Moreover, C/EBPα is not vital for adipocyte survival in the adult stage. We reveal a surprising diversity of transcriptional signals required at different stages of adipogenesis in vivo.

Full Text

Duke Authors

Cited Authors

  • Wang, QA; Tao, C; Jiang, L; Shao, M; Ye, R; Zhu, Y; Gordillo, R; Ali, A; Lian, Y; Holland, WL; Gupta, RK; Scherer, PE

Published Date

  • September 2015

Published In

Volume / Issue

  • 17 / 9

Start / End Page

  • 1099 - 1111

PubMed ID

  • 26280538

Pubmed Central ID

  • PMC4553131

Electronic International Standard Serial Number (EISSN)

  • 1476-4679

Digital Object Identifier (DOI)

  • 10.1038/ncb3217

Language

  • eng

Conference Location

  • England