Regulation of AUXIN RESPONSE FACTOR condensation and nucleo-cytoplasmic partitioning.

Journal Article (Journal Article)

Auxin critically regulates plant growth and development. Auxin-driven transcriptional responses are mediated through the AUXIN RESPONSE FACTOR (ARF) family of transcription factors. ARF protein condensation attenuates ARF activity, resulting in dramatic shifts in the auxin transcriptional landscape. Here, we perform a forward genetics screen for ARF hypercondensation, identifying an F-box protein, which we named AUXIN RESPONSE FACTOR F-BOX1 (AFF1). Functional characterization of SCFAFF1 revealed that this E3 ubiquitin ligase directly interacts with ARF19 and ARF7 to regulate their accumulation, condensation, and nucleo-cytoplasmic partitioning. Mutants defective in AFF1 display attenuated auxin responsiveness, and developmental defects, suggesting that SCFAFF1 -mediated regulation of ARF protein drives aspects of auxin response and plant development.

Full Text

Duke Authors

Cited Authors

  • Jing, H; Korasick, DA; Emenecker, RJ; Morffy, N; Wilkinson, EG; Powers, SK; Strader, LC

Published Date

  • July 2022

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 4015 -

PubMed ID

  • 35817767

Pubmed Central ID

  • PMC9273615

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-022-31628-2

Language

  • eng