Engineering consortia by polymeric microbial swarmbots.

Journal Article (Journal Article)

Synthetic microbial consortia represent a new frontier for synthetic biology given that they can solve more complex problems than monocultures. However, most attempts to co-cultivate these artificial communities fail because of the winner-takes-all in nutrients competition. In soil, multiple species can coexist with a spatial organization. Inspired by nature, here we show that an engineered spatial segregation method can assemble stable consortia with both flexibility and precision. We create microbial swarmbot consortia (MSBC) by encapsulating subpopulations with polymeric microcapsules. The crosslinked structure of microcapsules fences microbes, but allows the transport of small molecules and proteins. MSBC method enables the assembly of various synthetic communities and the precise control over the subpopulations. These capabilities can readily modulate the division of labor and communication. Our work integrates the synthetic biology and material science to offer insights into consortia assembly and serve as foundation to diverse applications from biomanufacturing to engineered photosynthesis.

Full Text

Duke Authors

Cited Authors

  • Wang, L; Zhang, X; Tang, C; Li, P; Zhu, R; Sun, J; Zhang, Y; Cui, H; Ma, J; Song, X; Zhang, W; Gao, X; Luo, X; You, L; Chen, Y; Dai, Z

Published Date

  • July 2022

Published In

Volume / Issue

  • 13 / 1

Start / End Page

  • 3879 -

PubMed ID

  • 35790722

Pubmed Central ID

  • PMC9256712

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

International Standard Serial Number (ISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-022-31467-1

Language

  • eng