Particle hydrogels decrease cerebral atrophy and attenuate astrocyte and microglia/macrophage reactivity after stroke.

Journal Article (Journal Article)

Increasing numbers of individuals live with stroke related disabilities. Following stroke, highly reactive astrocytes and pro-inflammatory microglia can release cytokines and lead to a cytotoxic environment that causes further brain damage and prevents endogenous repair. Paradoxically, these same cells also activate pro-repair mechanisms that contribute to endogenous repair and brain plasticity. Here, we show that the direct injection of a hyaluronic acid based microporous annealed particle (MAP) hydrogel into the stroke core in mice reduces the percent of highly reactive astrocytes, increases the percent of alternatively activated microglia, decreases cerebral atrophy and preserves NF200 axonal bundles. Further, we show that MAP hydrogel promotes reparative astrocyte infiltration into the lesion, which directly coincides with axonal penetration into the lesion. This work shows that the injection of a porous scaffold into the stroke core can lead to clinically relevant decrease in cerebral atrophy and modulates astrocytes and microglia towards a pro-repair phenotype.

Full Text

Duke Authors

Cited Authors

  • Sideris, E; Kioulaphides, S; Wilson, K; Yu, A; Chen, J; Carmichael, ST; Segura, T

Published Date

  • August 2022

Published In

  • Advanced Therapeutics

Volume / Issue

  • 5 / 8

Start / End Page

  • 2200048 -

PubMed ID

  • 36589207

Pubmed Central ID

  • PMC9797126

Electronic International Standard Serial Number (EISSN)

  • 2366-3987

International Standard Serial Number (ISSN)

  • 2366-3987

Digital Object Identifier (DOI)

  • 10.1002/adtp.202200048

Language

  • eng