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Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27.

Publication ,  Journal Article
Wan, M; Huang, W; Kute, TE; Miller, LD; Zhang, Q; Hatcher, H; Wang, J; Stovall, DB; Russell, GB; Cao, PD; Deng, Z; Wang, W; Zhang, Q; Lei, M ...
Published in: Am J Pathol
May 2012

Yin Yang 1 (YY1) is highly expressed in various types of cancers and regulates tumorigenesis through multiple pathways. In the present study, we evaluated YY1 expression levels in breast cancer cell lines, a breast cancer TMA, and two gene arrays. We observed that, compared with normal samples, YY1 is generally overexpressed in breast cancer cells and tissues. In functional studies, depletion of YY1 inhibited the clonogenicity, migration, invasion, and tumor formation of breast cancer cells, but did not affect the clonogenicity of nontumorigenic cells. Conversely, ectopically expressed YY1 enhanced the migration and invasion of nontumorigenic MCF-10A breast cells. In both a monolayer culture condition and a three-dimensional Matrigel system, silenced YY1 expression changed the architecture of breast cancer MCF-7 cells to that resembling MCF-10A cells, whereas ectopically expressed YY1 in MCF-10A cells had the opposite effect. Furthermore, we detected an inverse correlation between YY1 and p27 expression in both breast cancer cells and xenograft tumors with manipulated YY1 expression. Counteracting the changes in p27 expression attenuated the effects of YY1 alterations on these cells. In addition, YY1 promoted p27 ubiquitination and physically interacted with p27. In conclusion, our data suggest that YY1 is an oncogene and identify p27 as a new target of YY1.

Published In

Am J Pathol

DOI

EISSN

1525-2191

Publication Date

May 2012

Volume

180

Issue

5

Start / End Page

2120 / 2133

Location

United States

Related Subject Headings

  • YY1 Transcription Factor
  • Up-Regulation
  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Real-Time Polymerase Chain Reaction
  • Protein Processing, Post-Translational
  • Proliferating Cell Nuclear Antigen
  • Pathology
  • Oligonucleotide Array Sequence Analysis
  • Neoplastic Stem Cells
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wan, M., Huang, W., Kute, T. E., Miller, L. D., Zhang, Q., Hatcher, H., … Sui, G. (2012). Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27. Am J Pathol, 180(5), 2120–2133. https://doi.org/10.1016/j.ajpath.2012.01.037
Wan, Meimei, Weiwei Huang, Timothy E. Kute, Lance D. Miller, Qiang Zhang, Heather Hatcher, Jingxuan Wang, et al. “Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27.Am J Pathol 180, no. 5 (May 2012): 2120–33. https://doi.org/10.1016/j.ajpath.2012.01.037.
Wan M, Huang W, Kute TE, Miller LD, Zhang Q, Hatcher H, et al. Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27. Am J Pathol. 2012 May;180(5):2120–33.
Wan, Meimei, et al. “Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27.Am J Pathol, vol. 180, no. 5, May 2012, pp. 2120–33. Pubmed, doi:10.1016/j.ajpath.2012.01.037.
Wan M, Huang W, Kute TE, Miller LD, Zhang Q, Hatcher H, Wang J, Stovall DB, Russell GB, Cao PD, Deng Z, Wang W, Lei M, Torti SV, Akman SA, Sui G. Yin Yang 1 plays an essential role in breast cancer and negatively regulates p27. Am J Pathol. 2012 May;180(5):2120–2133.
Journal cover image

Published In

Am J Pathol

DOI

EISSN

1525-2191

Publication Date

May 2012

Volume

180

Issue

5

Start / End Page

2120 / 2133

Location

United States

Related Subject Headings

  • YY1 Transcription Factor
  • Up-Regulation
  • Tumor Cells, Cultured
  • Transplantation, Heterologous
  • Real-Time Polymerase Chain Reaction
  • Protein Processing, Post-Translational
  • Proliferating Cell Nuclear Antigen
  • Pathology
  • Oligonucleotide Array Sequence Analysis
  • Neoplastic Stem Cells