A hot spot for hydrogen peroxide-induced damage in the human hypoxia-inducible factor 1 binding site of the PGK 1 gene.

Journal Article (Journal Article)

Using ligation-mediated polymerase chain reaction to separately map the distribution of induced oxidized bases and strand breaks along the human PGK1 promoter at nucleotide resolution, we previously described the pattern of oxidative DNA damage induced in vitro by Cu(II)/ascorbate/H2O2 [J. Biol. Chem. 270, 17633-17640 (1995)]. Here we report that the pattern of in vivo base damage caused by H2O2 is almost identical to that of the previously used in vitro system with the exception of transcription factor-associated footprints. An unusually strong positive footprint for both strand breaks and oxidized bases is associated with binding of the hypoxia-inducible transcription factor-1. Base damage at this footprint was 52-91% repaired in 24 h, which was similar to the global base damage repair rate. However, strand breaks at this footprint were only 39-55% repaired in 24 h or approximately 100-fold slower than the global strand break repair rate.

Full Text

Duke Authors

Cited Authors

  • Rodriguez, H; Drouin, R; Holmquist, GP; Akman, SA

Published Date

  • February 15, 1997

Published In

Volume / Issue

  • 338 / 2

Start / End Page

  • 207 - 212

PubMed ID

  • 9028873

International Standard Serial Number (ISSN)

  • 0003-9861

Digital Object Identifier (DOI)

  • 10.1006/abbi.1996.9820


  • eng

Conference Location

  • United States