Differential pulse polarographic determination of plasma menadione.

Journal Article (Journal Article)

A differential pulse polarographic assay for plasma vitamin K3 (menadione) has been developed. Details of the assay are (i) lipid-soluble material is extracted from plasma into ether by the method of Bjornsson et al. [(1978) Thromb. Haemostas. 2, 466-473]; (ii) ether is evaporated under nitrogen and the residue is dissolved in the supporting electrolyte, methanol: 0.2 M borate buffer (9:1), pH 6.8; (iii) current height is measured at -0.32 V vs SCE on the differential pulse polarogram. The lower sensitivity limit of this technique after addition of standard vitamin K3 to plasma is 0.3 microM; the calibration curve is linear from 0.6 through 10 microM. Two patients treated with a single dose of menadiol sodium diphosphate, 20 mg/M2 i.m., achieved measurable plasma vitamin K3 levels at 0.5 to 1.0 h ranging between 0.5 (0.08 micrograms/ml) and 2 microM (0.3 micrograms/ml).

Full Text

Duke Authors

Cited Authors

  • Akman, SA; Kusu, F; Takamura, K; Chlebowski, R; Block, J

Published Date

  • September 1, 1984

Published In

Volume / Issue

  • 141 / 2

Start / End Page

  • 488 - 493

PubMed ID

  • 6496951

International Standard Serial Number (ISSN)

  • 0003-2697

Digital Object Identifier (DOI)

  • 10.1016/0003-2697(84)90075-7


  • eng

Conference Location

  • United States