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The identification and characterization of a G4-DNA resolvase activity.

Publication ,  Journal Article
Harrington, C; Lan, Y; Akman, SA
Published in: The Journal of biological chemistry
September 1997

There is increasing evidence that four-stranded Hoogsteen-bonded DNA structures, G4-DNA, play an important role in cellular processes such as meiosis and recombination. The Hoogsteen-bonded G4-DNA is thermodynamically more stable than duplex DNA, and many guanine-rich genomic DNA sequences with the ability to form G4-DNA have been identified. A protein-dependent activity that resolves G4-DNA into single-stranded DNA has been identified in human placental tissue. The resolvase activity was purified from any apparent nuclease activity and is dependent on NTP hydrolysis and MgCl2. Resolvase activity is optimal with 5 mM MgCl2. The Vmax/Km of ATP is 0. 055%/min/microM, higher than the Vmax/Km of the other dNTPs. The products of the resolvase reaction are unmodified single-stranded DNA. The resolvase is not a duplex DNA helicase or a topoisomerase II activity and does not unwind Hoogsteen-bonded triplex DNA. Resolvase is a novel activity that unwinds stable G4-DNA structures using a dNTP-dependent mechanism producing unmodified single-stranded DNA. Potential in vivo roles for this G4-DNA resolvase activity are discussed.

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Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

September 1997

Volume

272

Issue

39

Start / End Page

24631 / 24636

Related Subject Headings

  • Transposases
  • Nucleotides
  • Magnesium Chloride
  • Kinetics
  • Hydrolysis
  • Humans
  • Hot Temperature
  • DNA, Single-Stranded
  • DNA Topoisomerases, Type II
  • DNA Nucleotidyltransferases
 

Citation

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Harrington, C., Lan, Y., & Akman, S. A. (1997). The identification and characterization of a G4-DNA resolvase activity. The Journal of Biological Chemistry, 272(39), 24631–24636. https://doi.org/10.1074/jbc.272.39.24631
Harrington, C., Y. Lan, and S. A. Akman. “The identification and characterization of a G4-DNA resolvase activity.The Journal of Biological Chemistry 272, no. 39 (September 1997): 24631–36. https://doi.org/10.1074/jbc.272.39.24631.
Harrington C, Lan Y, Akman SA. The identification and characterization of a G4-DNA resolvase activity. The Journal of biological chemistry. 1997 Sep;272(39):24631–6.
Harrington, C., et al. “The identification and characterization of a G4-DNA resolvase activity.The Journal of Biological Chemistry, vol. 272, no. 39, Sept. 1997, pp. 24631–36. Epmc, doi:10.1074/jbc.272.39.24631.
Harrington C, Lan Y, Akman SA. The identification and characterization of a G4-DNA resolvase activity. The Journal of biological chemistry. 1997 Sep;272(39):24631–24636.

Published In

The Journal of biological chemistry

DOI

EISSN

1083-351X

ISSN

0021-9258

Publication Date

September 1997

Volume

272

Issue

39

Start / End Page

24631 / 24636

Related Subject Headings

  • Transposases
  • Nucleotides
  • Magnesium Chloride
  • Kinetics
  • Hydrolysis
  • Humans
  • Hot Temperature
  • DNA, Single-Stranded
  • DNA Topoisomerases, Type II
  • DNA Nucleotidyltransferases