Rat liver NAD(P)H:quinone oxidoreductase: cDNA expression and site-directed mutagenesis.

Journal Article (Journal Article)

Rat liver NAD(P)H:quinone oxidoreductase cDNA was cloned and expressed in a eukaryotic cell expression plasmid containing a cytomegalovirus (CMV) promoter. Transient expression of enzyme activity and RNA transcription were measured in COS7 cells. The expressed quinone reductase has kinetic properties similar to the rat liver enzyme and is inhibited by dicourmarol, a known inhibitor of NAD(P)H:quinone oxidoreductase. Site-directed mutagenesis experiments carried out using this expression system revealed possible regions involved in NAD(P)H binding.

Full Text

Duke Authors

Cited Authors

  • Forrest, GL; Qian, J; Ma, JX; Kaplan, WD; Akman, S; Doroshow, J; Chen, SA

Published Date

  • June 29, 1990

Published In

Volume / Issue

  • 169 / 3

Start / End Page

  • 1087 - 1093

PubMed ID

  • 2141979

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(90)92006-l


  • eng

Conference Location

  • United States