Induction of a human carbonyl reductase gene located on chromosome 21.

Journal Article (Journal Article)

Carbonyl reductase (EC belongs to the group of enzymes called aldo-keto reductases. It is a NADPH-dependent cytosolic protein with specificity for many carbonyl compounds including the antitumor anthracycline antibiotics, daunorubicin and doxorubicin. Human carbonyl reductase was cloned from a breast cancer cell line (MCF-7). The cDNA clone contained 1219 base paires with an open reading frame corresponding to 277 amino acids encoding a protein of Mr 30,375. Southern analysis of genomic DNA digested with several restriction enzymes and analyzed by hybridization with a labeled cDNA probe indicated that carbonyl reductase is probably coded by a single gene and does not belong to a family of structurally similar enzymes. Southern analysis of 17 mouse/human somatic cell hybrids showed that carbonyl reductase is located on chromosome 21. Carbonyl reductase mRNA could be induced 3-4-fold in 24 h with 10 microM 2,(3)-t-butyl-4-hydroxyanisole (BHA), beta-naphthoflavone or Sudan 1.

Full Text

Duke Authors

Cited Authors

  • Forrest, GL; Akman, S; Krutzik, S; Paxton, RJ; Sparkes, RS; Doroshow, J; Felsted, RL; Glover, CJ; Mohandas, T; Bachur, NR

Published Date

  • April 6, 1990

Published In

Volume / Issue

  • 1048 / 2-3

Start / End Page

  • 149 - 155

PubMed ID

  • 2182121

International Standard Serial Number (ISSN)

  • 0006-3002

Digital Object Identifier (DOI)

  • 10.1016/0167-4781(90)90050-c


  • eng

Conference Location

  • Netherlands