Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription.

Journal Article (Journal Article)

Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S. cerevesiae that sense changes in the cellular environment and repress pol III transcription. Signaling pathways activated in response to rapamycin-induced nutrient limitation, DNA damage, and secretory pathway defects all require Maf1 in order to affect pol III transcriptional repression. In addition, Maf1 was required for repression of pol III transcription during the normal yeast growth cycle. Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.

Full Text

Duke Authors

Cited Authors

  • Upadhya, R; Lee, J; Willis, IM

Published Date

  • December 2002

Published In

Volume / Issue

  • 10 / 6

Start / End Page

  • 1489 - 1494

PubMed ID

  • 12504022

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/s1097-2765(02)00787-6

Language

  • eng

Conference Location

  • United States