Skip to main content
Journal cover image

Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery.

Publication ,  Journal Article
Santagata, S; Eberlin, LS; Norton, I; Calligaris, D; Feldman, DR; Ide, JL; Liu, X; Wiley, JS; Vestal, ML; Ramkissoon, SH; Orringer, DA ...
Published in: Proc Natl Acad Sci U S A
July 29, 2014

For many intraoperative decisions surgeons depend on frozen section pathology, a technique developed over 150 y ago. Technical innovations that permit rapid molecular characterization of tissue samples at the time of surgery are needed. Here, using desorption electrospray ionization (DESI) MS, we rapidly detect the tumor metabolite 2-hydroxyglutarate (2-HG) from tissue sections of surgically resected gliomas, under ambient conditions and without complex or time-consuming preparation. With DESI MS, we identify isocitrate dehydrogenase 1-mutant tumors with both high sensitivity and specificity within minutes, immediately providing critical diagnostic, prognostic, and predictive information. Imaging tissue sections with DESI MS shows that the 2-HG signal overlaps with areas of tumor and that 2-HG levels correlate with tumor content, thereby indicating tumor margins. Mapping the 2-HG signal onto 3D MRI reconstructions of tumors allows the integration of molecular and radiologic information for enhanced clinical decision making. We also validate the methodology and its deployment in the operating room: We have installed a mass spectrometer in our Advanced Multimodality Image Guided Operating (AMIGO) suite and demonstrate the molecular analysis of surgical tissue during brain surgery. This work indicates that metabolite-imaging MS could transform many aspects of surgical care.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

July 29, 2014

Volume

111

Issue

30

Start / End Page

11121 / 11126

Location

United States

Related Subject Headings

  • Radiography
  • Mass Spectrometry
  • Male
  • Magnetic Resonance Imaging
  • Intraoperative Care
  • Humans
  • Glutarates
  • Glioma
  • Female
  • Brain Neoplasms
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Santagata, S., Eberlin, L. S., Norton, I., Calligaris, D., Feldman, D. R., Ide, J. L., … Agar, N. Y. R. (2014). Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery. Proc Natl Acad Sci U S A, 111(30), 11121–11126. https://doi.org/10.1073/pnas.1404724111
Santagata, Sandro, Livia S. Eberlin, Isaiah Norton, David Calligaris, Daniel R. Feldman, Jennifer L. Ide, Xiaohui Liu, et al. “Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery.Proc Natl Acad Sci U S A 111, no. 30 (July 29, 2014): 11121–26. https://doi.org/10.1073/pnas.1404724111.
Santagata S, Eberlin LS, Norton I, Calligaris D, Feldman DR, Ide JL, et al. Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery. Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):11121–6.
Santagata, Sandro, et al. “Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery.Proc Natl Acad Sci U S A, vol. 111, no. 30, July 2014, pp. 11121–26. Pubmed, doi:10.1073/pnas.1404724111.
Santagata S, Eberlin LS, Norton I, Calligaris D, Feldman DR, Ide JL, Liu X, Wiley JS, Vestal ML, Ramkissoon SH, Orringer DA, Gill KK, Dunn IF, Dias-Santagata D, Ligon KL, Jolesz FA, Golby AJ, Cooks RG, Agar NYR. Intraoperative mass spectrometry mapping of an onco-metabolite to guide brain tumor surgery. Proc Natl Acad Sci U S A. 2014 Jul 29;111(30):11121–11126.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

EISSN

1091-6490

Publication Date

July 29, 2014

Volume

111

Issue

30

Start / End Page

11121 / 11126

Location

United States

Related Subject Headings

  • Radiography
  • Mass Spectrometry
  • Male
  • Magnetic Resonance Imaging
  • Intraoperative Care
  • Humans
  • Glutarates
  • Glioma
  • Female
  • Brain Neoplasms