African and Asian strains of Zika virus differ in their ability to infect and lyse primitive human placental trophoblast.

Journal Article (Journal Article)

Zika virus (ZIKV) drew worldwide attention when a recent epidemic was linked to fetal microcephaly. Here we used human embryonic stem cell derived trophoblasts as a model for primitive placental trophoblast to test the hypothesis that there are differences in how the two genetically distinct ZIKV lineages, African (AF) and Asian (AS), target the human placenta. Upon infection with three AF (ib-H30656, SEN/1984/41525-DAK, and MR-766) and three AS (FSS13025, MexI-44, and PANcdc259249) ZIKV strains, we observed that severe placental cell lysis was only induced after infection with AF strains, while viral replication rates remained similar between both lineages. Differences in cytopathic effects (CPE) were not observed in Vero cells, indicating that the AF strains were not inherently superior at cell lysis. Taken together, we propose that infection with AF strains of ZIKV early in pregnancy would likely result in pregnancy loss, rather than allow further fetal development with accompanying brain damage. Our results also suggest that the long term laboratory-adapted MR-766 strain does not behave aberrantly in cell culture relative to other AF lineage strains.

Full Text

Duke Authors

Cited Authors

  • Sheridan, MA; Balaraman, V; Schust, DJ; Ezashi, T; Roberts, RM; Franz, AWE

Published Date

  • 2018

Published In

Volume / Issue

  • 13 / 7

Start / End Page

  • e0200086 -

PubMed ID

  • 29985932

Pubmed Central ID

  • PMC6037361

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0200086


  • eng

Conference Location

  • United States