Differentiation of trophoblast cells from human embryonic stem cells: to be or not to be?

Journal Article (Journal Article)

It is imperative to unveil the full range of differentiated cell types into which human pluripotent stem cells (hPSCs) can develop. The need is twofold: it will delimit the therapeutic utility of these stem cells and is necessary to place their position accurately in the developmental hierarchy of lineage potential. Accumulated evidence suggested that hPSC could develop in vitro into an extraembryonic lineage (trophoblast (TB)) that is typically inaccessible to pluripotent embryonic cells during embryogenesis. However, whether these differentiated cells are truly authentic TB has been challenged. In this debate, we present a case for and a case against TB differentiation from hPSCs. By analogy to other differentiation systems, our debate is broadly applicable, as it articulates higher and more challenging standards for judging whether a given cell type has been genuinely produced from hPSC differentiation.

Full Text

Duke Authors

Cited Authors

  • Roberts, RM; Loh, KM; Amita, M; Bernardo, AS; Adachi, K; Alexenko, AP; Schust, DJ; Schulz, LC; Telugu, BPVL; Ezashi, T; Pedersen, RA

Published Date

  • May 2014

Published In

Volume / Issue

  • 147 / 5

Start / End Page

  • D1 - 12

PubMed ID

  • 24518070

Electronic International Standard Serial Number (EISSN)

  • 1741-7899

Digital Object Identifier (DOI)

  • 10.1530/REP-14-0080

Language

  • eng

Conference Location

  • England