A possible coagulation-independent mechanism for pregnancy loss involving β(2) glycoprotein 1-dependent antiphospholipid antibodies and CD1d.

Journal Article (Journal Article)

PROBLEM  β(2) glycoprotein1 (β(2) GP1)-dependent antiphospholipid antibodies (aPL) increase the risk for recurrent pregnancy loss. We address whether anti-β(2) GP1 antibodies can interact with phosphatidylserine (PS)-bearing CD1d on trophoblast cells and induce local inflammation. METHODS  CD1d-bearing choriocarcinoma cells were used in flow cytometry and immunoprecipitation experiments. CD1d-mediated cytokine induction was assessed using antibody cross-linking. Cytokine production during co-culture of decidual lymphocytes with CD1d-bearing cells was also examined. RESULTS  Trophoblast surface-expressed CD1d forms a complex with PS-bound β(2) GP1. Anti-β(2) GP1 mAb cross-linking causes IL12p70 release from CD1d-bearing cells. IL12p70 release from CD1d-bearing trophoblast cells was also induced during co-culture with human decidual lymphocytes. The addition of anti-β2GP1 mAb to co-cultures resulted in a three-fold increase in IL12p70 secretion. IFNγ secretion from decidual lymphocytes was also induced during co-culture with anti-β2GP1 mAbs. CONCLUSIONS  β(2) GP1-dependent IL12 release from CD1d-bearing trophoblast in the presence of aPL may link the antiphospholipid syndrome to pregnancy loss via an inflammatory mechanism.

Full Text

Duke Authors

Cited Authors

  • Iwasawa, Y; Kawana, K; Fujii, T; Schust, DJ; Nagamatsu, T; Kawana, Y; Sayama, S; Miura, S; Matsumoto, J; Adachi, K; Hyodo, H; Yamashita, T; Kozuma, S; Taketani, Y

Published Date

  • January 2012

Published In

Volume / Issue

  • 67 / 1

Start / End Page

  • 54 - 65

PubMed ID

  • 21682789

Electronic International Standard Serial Number (EISSN)

  • 1600-0897

Digital Object Identifier (DOI)

  • 10.1111/j.1600-0897.2011.01028.x

Language

  • eng

Conference Location

  • Denmark