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Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development.

Publication ,  Journal Article
Kam, Y-W; Lee, WWL; Simarmata, D; Harjanto, S; Teng, T-S; Tolou, H; Chow, A; Lin, RTP; Leo, Y-S; Rénia, L; Ng, LFP
Published in: Journal of virology
December 2012

Chikungunya virus (CHIKV) is an alphavirus which causes chronic and incapacitating arthralgia in humans. Although previous studies have shown that antibodies against the virus are produced during and after infection, the fine specificity of the antibody response against CHIKV is not known. Here, using plasma from patients at different times postinfection, we characterized the antibody response against various proteins of the virus. We have shown that the E2 and E3 glycoproteins and the capsid and nsP3 proteins are targets of the anti-CHIKV antibody response. Moreover, we have identified the different regions in these proteins which contain the linear epitopes recognized by the anti-CHIKV antibodies and determined their structural localization. Data also illustrated the effect of a single K(252)Q amino acid change at the E2 glycoprotein that was able to influence antibody binding and interaction between the antibodies and epitope because of the changes of epitope-antibody binding capacity. This study provides important knowledge that will not only aid in the understanding of the immune response to CHIKV infection but also provide new knowledge in the design of modern vaccine development. Furthermore, these pathogen-specific epitopes could be used for future seroepidemiological studies that will unravel the molecular mechanisms of human immunity and protection from CHIKV disease.

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Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

December 2012

Volume

86

Issue

23

Start / End Page

13005 / 13015

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Proteins
  • Singapore
  • Serologic Tests
  • Models, Biological
  • Longitudinal Studies
  • Immunoblotting
  • Humans
  • HEK293 Cells
 

Citation

APA
Chicago
ICMJE
MLA
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Kam, Y.-W., Lee, W. W. L., Simarmata, D., Harjanto, S., Teng, T.-S., Tolou, H., … Ng, L. F. P. (2012). Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development. Journal of Virology, 86(23), 13005–13015. https://doi.org/10.1128/jvi.01780-12
Kam, Yiu-Wing, Wendy W. L. Lee, Diane Simarmata, Sumitro Harjanto, Terk-Shin Teng, Hugues Tolou, Angela Chow, et al. “Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development.Journal of Virology 86, no. 23 (December 2012): 13005–15. https://doi.org/10.1128/jvi.01780-12.
Kam Y-W, Lee WWL, Simarmata D, Harjanto S, Teng T-S, Tolou H, et al. Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development. Journal of virology. 2012 Dec;86(23):13005–15.
Kam, Yiu-Wing, et al. “Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development.Journal of Virology, vol. 86, no. 23, Dec. 2012, pp. 13005–15. Epmc, doi:10.1128/jvi.01780-12.
Kam Y-W, Lee WWL, Simarmata D, Harjanto S, Teng T-S, Tolou H, Chow A, Lin RTP, Leo Y-S, Rénia L, Ng LFP. Longitudinal analysis of the human antibody response to Chikungunya virus infection: implications for serodiagnosis and vaccine development. Journal of virology. 2012 Dec;86(23):13005–13015.

Published In

Journal of virology

DOI

EISSN

1098-5514

ISSN

0022-538X

Publication Date

December 2012

Volume

86

Issue

23

Start / End Page

13005 / 13015

Related Subject Headings

  • Virology
  • Viral Vaccines
  • Viral Proteins
  • Singapore
  • Serologic Tests
  • Models, Biological
  • Longitudinal Studies
  • Immunoblotting
  • Humans
  • HEK293 Cells