An engineered ScCas9 with broad PAM range and high specificity and activity.

Journal Article (Journal Article)

CRISPR enzymes require a protospacer-adjacent motif (PAM) near the target cleavage site, constraining the sequences accessible for editing. In the present study, we combine protein motifs from several orthologs to engineer two variants of Streptococcus canis Cas9-Sc++ and a higher-fidelity mutant HiFi-Sc++ -that have simultaneously broad 5'-NNG-3' PAM compatibility, robust DNA-cleavage activity and minimal off-target activity. Sc++ and HiFi-Sc++ extend the use of CRISPR editing for diverse applications.

Full Text

Duke Authors

Cited Authors

  • Chatterjee, P; Jakimo, N; Lee, J; Amrani, N; Rodríguez, T; Koseki, SRT; Tysinger, E; Qing, R; Hao, S; Sontheimer, EJ; Jacobson, J

Published Date

  • October 2020

Published In

Volume / Issue

  • 38 / 10

Start / End Page

  • 1154 - 1158

PubMed ID

  • 32393822

Electronic International Standard Serial Number (EISSN)

  • 1546-1696

International Standard Serial Number (ISSN)

  • 1087-0156

Digital Object Identifier (DOI)

  • 10.1038/s41587-020-0517-0

Language

  • eng