Determination of TE Insertion Positions Using Transposon Display.
Publication
, Chapter
Kim, EY; Fan, W; Cho, J
January 2021
Mapping the genomic location to which transposons jumped is of greatest interest to transposon biologists. Transposon display (TD) is the technique of choice that is easy and fast in determining the neo-insertion positions of a target transposon. Essentially, tagging of transposon is performed by digesting genomic DNA, ligating adaptors to digested DNA ends and PCR amplifying genomic regions flanking the transposon of interest. In this chapter, the experimental procedure of TD is described using Onsen retrotransposon of Arabidopsis as an example.
Duke Scholars
DOI
Publication Date
January 2021
Volume
2250
Start / End Page
115 / 121
Related Subject Headings
- Retroelements
- Mutagenesis, Insertional
- Developmental Biology
- DNA, Plant
- Chromosome Mapping
- Arabidopsis
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Kim, E. Y., Fan, W., & Cho, J. (2021). Determination of TE Insertion Positions Using Transposon Display. (Vol. 2250, pp. 115–121). https://doi.org/10.1007/978-1-0716-1134-0_11
Kim, Eun Yu, Wenwen Fan, and Jungnam Cho. “Determination of TE Insertion Positions Using Transposon Display.,” 2250:115–21, 2021. https://doi.org/10.1007/978-1-0716-1134-0_11.
Kim EY, Fan W, Cho J. Determination of TE Insertion Positions Using Transposon Display. In 2021. p. 115–21.
Kim, Eun Yu, et al. Determination of TE Insertion Positions Using Transposon Display. Vol. 2250, 2021, pp. 115–21. Epmc, doi:10.1007/978-1-0716-1134-0_11.
Kim EY, Fan W, Cho J. Determination of TE Insertion Positions Using Transposon Display. 2021. p. 115–121.
DOI
Publication Date
January 2021
Volume
2250
Start / End Page
115 / 121
Related Subject Headings
- Retroelements
- Mutagenesis, Insertional
- Developmental Biology
- DNA, Plant
- Chromosome Mapping
- Arabidopsis
- 3404 Medicinal and biomolecular chemistry
- 3101 Biochemistry and cell biology
- 0601 Biochemistry and Cell Biology
- 0399 Other Chemical Sciences