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The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability.

Publication ,  Journal Article
Seo, YS; Kim, EY; Kim, WT
Published in: Journal of experimental botany
November 2011

Lipid-derived molecules produced by acylhydrolases play important roles in the regulation of diverse cellular functions in plants. In Arabidopsis, the DAD1-like phospholipase A1 family consists of 12 members, all of which possess a lipase 3 domain. In this study, the biochemical and cellular functions of AtDLAH, an Arabidopsis thaliana DAD1-like acylhydrolase, were examined. Bacterially expressed AtDLAH contained phospholipase A1 activity for catalysing the hydrolysis of phospholipids at the sn-1 position. However, AtDLAH displayed an even stronger preference for 1-lysophosphatidylcholine, 1-monodiacylglycerol, and phosphatidic acid, suggesting that AtDLAH is a sn-1-specific acylhydrolase. The AtDLAH gene was highly expressed in young seedlings, and its encoded protein was exclusively localized to the mitochondria. AtDLAH-overexpressing transgenic seeds (35S:AtDLAH) were markedly tolerant to accelerated-ageing treatment and thus had higher germination percentages than wild-type seeds. In contrast, the atdlah loss-of-function knockout mutant seeds were hypersusceptible to accelerated-ageing conditions. The 35S:AtDLAH seeds, as opposed to the atdlah seeds, exhibited a dark red staining pattern following tetrazolium treatment under both normal and accelerated-ageing conditions, suggesting that AtDLAH expression is positively correlated with seed viability. The enhanced viability of 35S:AtDLAH seeds was accompanied by more densely populated epidermal cells, lower levels of accumulated lipid hydroperoxides, and higher levels of polar lipids as compared with wild-type and atdlah mutant seeds. These results suggest that AtDLAH, a mitochondrial-localized sn-1-specific acylhydrolase, plays an important role in Arabidopsis seed viability.

Duke Scholars

Published In

Journal of experimental botany

DOI

EISSN

1460-2431

ISSN

0022-0957

Publication Date

November 2011

Volume

62

Issue

15

Start / End Page

5683 / 5698

Related Subject Headings

  • Seeds
  • Plant Biology & Botany
  • Mitochondrial Proteins
  • Microscopy, Electron, Scanning
  • Lipid Peroxidation
  • Gene Expression Regulation, Plant
  • Chromatography, Thin Layer
  • Arabidopsis Proteins
  • Arabidopsis
  • 3108 Plant biology
 

Citation

APA
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MLA
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Seo, Y. S., Kim, E. Y., & Kim, W. T. (2011). The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability. Journal of Experimental Botany, 62(15), 5683–5698. https://doi.org/10.1093/jxb/err250
Seo, Young Sam, Eun Yu Kim, and Woo Taek Kim. “The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability.Journal of Experimental Botany 62, no. 15 (November 2011): 5683–98. https://doi.org/10.1093/jxb/err250.
Seo YS, Kim EY, Kim WT. The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability. Journal of experimental botany. 2011 Nov;62(15):5683–98.
Seo, Young Sam, et al. “The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability.Journal of Experimental Botany, vol. 62, no. 15, Nov. 2011, pp. 5683–98. Epmc, doi:10.1093/jxb/err250.
Seo YS, Kim EY, Kim WT. The Arabidopsis sn-1-specific mitochondrial acylhydrolase AtDLAH is positively correlated with seed viability. Journal of experimental botany. 2011 Nov;62(15):5683–5698.
Journal cover image

Published In

Journal of experimental botany

DOI

EISSN

1460-2431

ISSN

0022-0957

Publication Date

November 2011

Volume

62

Issue

15

Start / End Page

5683 / 5698

Related Subject Headings

  • Seeds
  • Plant Biology & Botany
  • Mitochondrial Proteins
  • Microscopy, Electron, Scanning
  • Lipid Peroxidation
  • Gene Expression Regulation, Plant
  • Chromatography, Thin Layer
  • Arabidopsis Proteins
  • Arabidopsis
  • 3108 Plant biology