Connectomics of the zebrafish's lateral-line neuromast reveals wiring and miswiring in a simple microcircuit.

Journal Article (Journal Article)

The lateral-line neuromast of the zebrafish displays a restricted, consistent pattern of innervation that facilitates the comparison of microcircuits across individuals, developmental stages, and genotypes. We used serial blockface scanning electron microscopy to determine from multiple specimens the neuromast connectome, a comprehensive set of connections between hair cells and afferent and efferent nerve fibers. This analysis delineated a complex but consistent wiring pattern with three striking characteristics: each nerve terminal is highly specific in receiving innervation from hair cells of a single directional sensitivity; the innervation is redundant; and the terminals manifest a hierarchy of dominance. Mutation of the canonical planar-cell-polarity gene vangl2, which decouples the asymmetric phenotypes of sibling hair-cell pairs, results in randomly positioned, randomly oriented sibling cells that nonetheless retain specific wiring. Because larvae that overexpress Notch exhibit uniformly oriented, uniformly innervating hair-cell siblings, wiring specificity is mediated by the Notch signaling pathway.

Full Text

Duke Authors

Cited Authors

  • Dow, E; Jacobo, A; Hossain, S; Siletti, K; Hudspeth, AJ

Published Date

  • June 12, 2018

Published In

Volume / Issue

  • 7 /

PubMed ID

  • 29893686

Pubmed Central ID

  • PMC5997450

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.33988

Language

  • eng

Conference Location

  • England