A robotics platform for automated batch fabrication of high density, microfluidics-based DNA microarrays, with applications to single cell, multiplex assays of secreted proteins.

Journal Article (Journal Article)

Microfluidics flow-patterning has been utilized for the construction of chip-scale miniaturized DNA and protein barcode arrays. Such arrays have been used for specific clinical and fundamental investigations in which many proteins are assayed from single cells or other small sample sizes. However, flow-patterned arrays are hand-prepared, and so are impractical for broad applications. We describe an integrated robotics/microfluidics platform for the automated preparation of such arrays, and we apply it to the batch fabrication of up to eighteen chips of flow-patterned DNA barcodes. The resulting substrates are comparable in quality with hand-made arrays and exhibit excellent substrate-to-substrate consistency. We demonstrate the utility and reproducibility of robotics-patterned barcodes by utilizing two flow-patterned chips for highly parallel assays of a panel of secreted proteins from single macrophage cells.

Full Text

Duke Authors

Cited Authors

  • Ahmad, H; Sutherland, A; Shin, YS; Hwang, K; Qin, L; Krom, R-J; Heath, JR

Published Date

  • September 2011

Published In

Volume / Issue

  • 82 / 9

Start / End Page

  • 094301 -

PubMed ID

  • 21974603

Pubmed Central ID

  • PMC3189969

Electronic International Standard Serial Number (EISSN)

  • 1089-7623

Digital Object Identifier (DOI)

  • 10.1063/1.3636077

Language

  • eng

Conference Location

  • United States