Iterative in situ click chemistry creates antibody-like protein-capture agents.

Journal Article (Journal Article)

Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.

Full Text

Duke Authors

Cited Authors

  • Agnew, HD; Rohde, RD; Millward, SW; Nag, A; Yeo, W-S; Hein, JE; Pitram, SM; Tariq, AA; Burns, VM; Krom, RJ; Fokin, VV; Sharpless, KB; Heath, JR

Published Date

  • 2009

Published In

Volume / Issue

  • 48 / 27

Start / End Page

  • 4944 - 4948

PubMed ID

  • 19301344

Pubmed Central ID

  • PMC3716464

Electronic International Standard Serial Number (EISSN)

  • 1521-3773

Digital Object Identifier (DOI)

  • 10.1002/anie.200900488


  • eng

Conference Location

  • Germany