Iterative in situ click chemistry creates antibody-like protein-capture agents.
Special agents for protein capture: Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one-bead-one-compound method for the creation of a peptide library enable the fragment-based assembly of selective high-affinity protein-capture agents. The resulting ligands are water-soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)-catalyzed cycloaddition.
Agnew, HD; Rohde, RD; Millward, SW; Nag, A; Yeo, W-S; Hein, JE; Pitram, SM; Tariq, AA; Burns, VM; Krom, RJ; Fokin, VV; Sharpless, KB; Heath, JR
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