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A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.

Publication ,  Journal Article
Lyu, Z; Park, J; Kim, K-M; Jin, H-J; Wu, H; Rajadas, J; Kim, D-H; Steinberg, GK; Lee, W
Published in: Nat Biomed Eng
August 2021

The therapeutic efficacy of stem cells transplanted into an ischaemic brain depends primarily on the responses of the neurovascular unit. Here, we report the development and applicability of a functional neurovascular unit on a microfluidic chip as a microphysiological model of ischaemic stroke that recapitulates the function of the blood-brain barrier as well as interactions between therapeutic stem cells and host cells (human brain microvascular endothelial cells, pericytes, astrocytes, microglia and neurons). We used the model to track the infiltration of a number of candidate stem cells and to characterize the expression levels of genes associated with post-stroke pathologies. We observed that each type of stem cell showed unique neurorestorative effects, primarily by supporting endogenous recovery rather than through direct cell replacement, and that the recovery of synaptic activities is correlated with the recovery of the structural and functional integrity of the neurovascular unit rather than with the regeneration of neurons.

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Published In

Nat Biomed Eng

DOI

EISSN

2157-846X

Publication Date

August 2021

Volume

5

Issue

8

Start / End Page

847 / 863

Location

England

Related Subject Headings

  • Stem Cells
  • Stem Cell Transplantation
  • Pericytes
  • Neurons
  • Models, Biological
  • Microvessels
  • Microglia
  • Lab-On-A-Chip Devices
  • Ischemic Stroke
  • Humans
 

Citation

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MLA
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Lyu, Z., Park, J., Kim, K.-M., Jin, H.-J., Wu, H., Rajadas, J., … Lee, W. (2021). A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke. Nat Biomed Eng, 5(8), 847–863. https://doi.org/10.1038/s41551-021-00744-7
Lyu, Zhonglin, Jon Park, Kwang-Min Kim, Hye-Jin Jin, Haodi Wu, Jayakumar Rajadas, Deok-Ho Kim, Gary K. Steinberg, and Wonjae Lee. “A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.Nat Biomed Eng 5, no. 8 (August 2021): 847–63. https://doi.org/10.1038/s41551-021-00744-7.
Lyu Z, Park J, Kim K-M, Jin H-J, Wu H, Rajadas J, et al. A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke. Nat Biomed Eng. 2021 Aug;5(8):847–63.
Lyu, Zhonglin, et al. “A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.Nat Biomed Eng, vol. 5, no. 8, Aug. 2021, pp. 847–63. Pubmed, doi:10.1038/s41551-021-00744-7.
Lyu Z, Park J, Kim K-M, Jin H-J, Wu H, Rajadas J, Kim D-H, Steinberg GK, Lee W. A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke. Nat Biomed Eng. 2021 Aug;5(8):847–863.

Published In

Nat Biomed Eng

DOI

EISSN

2157-846X

Publication Date

August 2021

Volume

5

Issue

8

Start / End Page

847 / 863

Location

England

Related Subject Headings

  • Stem Cells
  • Stem Cell Transplantation
  • Pericytes
  • Neurons
  • Models, Biological
  • Microvessels
  • Microglia
  • Lab-On-A-Chip Devices
  • Ischemic Stroke
  • Humans