A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.

Journal Article (Journal Article)

The therapeutic efficacy of stem cells transplanted into an ischaemic brain depends primarily on the responses of the neurovascular unit. Here, we report the development and applicability of a functional neurovascular unit on a microfluidic chip as a microphysiological model of ischaemic stroke that recapitulates the function of the blood-brain barrier as well as interactions between therapeutic stem cells and host cells (human brain microvascular endothelial cells, pericytes, astrocytes, microglia and neurons). We used the model to track the infiltration of a number of candidate stem cells and to characterize the expression levels of genes associated with post-stroke pathologies. We observed that each type of stem cell showed unique neurorestorative effects, primarily by supporting endogenous recovery rather than through direct cell replacement, and that the recovery of synaptic activities is correlated with the recovery of the structural and functional integrity of the neurovascular unit rather than with the regeneration of neurons.

Full Text

Duke Authors

Cited Authors

  • Lyu, Z; Park, J; Kim, K-M; Jin, H-J; Wu, H; Rajadas, J; Kim, D-H; Steinberg, GK; Lee, W

Published Date

  • August 2021

Published In

Volume / Issue

  • 5 / 8

Start / End Page

  • 847 - 863

PubMed ID

  • 34385693

Pubmed Central ID

  • PMC8524779

Electronic International Standard Serial Number (EISSN)

  • 2157-846X

Digital Object Identifier (DOI)

  • 10.1038/s41551-021-00744-7

Language

  • eng

Conference Location

  • England