A versatile system to record cell-cell interactions.

Journal Article (Journal Article)

Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation of the role of the cancer microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer to enable sensitive and specific labeling of cells after cell-cell interactions. G-baToN is a generalizable system that enables physical contact-based labeling between various human and mouse cell types, including endothelial cell-pericyte, neuron-astrocyte, and diverse cancer-stromal cell pairs. A suite of orthogonal baToN tools enables reciprocal cell-cell labeling, interaction-dependent cargo transfer, and the identification of higher order cell-cell interactions across a wide range of cell types. The ability to track physically interacting cells with these simple and sensitive systems will greatly accelerate our understanding of the outputs of cell-cell interactions in cancer as well as across many biological processes.

Full Text

Duke Authors

Cited Authors

  • Tang, R; Murray, CW; Linde, IL; Kramer, NJ; Lyu, Z; Tsai, MK; Chen, LC; Cai, H; Gitler, AD; Engleman, E; Lee, W; Winslow, MM

Published Date

  • October 7, 2020

Published In

Volume / Issue

  • 9 /

PubMed ID

  • 33025906

Pubmed Central ID

  • PMC7682987

Electronic International Standard Serial Number (EISSN)

  • 2050-084X

Digital Object Identifier (DOI)

  • 10.7554/eLife.61080

Language

  • eng

Conference Location

  • England