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Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery.

Publication ,  Journal Article
Green, WD; Alwarawrah, Y; Al-Shaer, AE; Shi, Q; Armstrong, M; Manke, J; Reisdorph, N; Farrell, TM; Hursting, SD; MacIver, NJ; Beck, MA; Shaikh, SR
Published in: J Infect Dis
December 28, 2022

BACKGROUND: Obesity dysregulates immunity to influenza infection. Therefore, there is a critical need to investigate how obesity impairs immunity and to establish therapeutic approaches that mitigate the impact of increased adiposity. One mechanism by which obesity may alter immune responses is through changes in cellular metabolism. METHODS: We studied inflammation and cellular metabolism of peripheral blood mononuclear cells (PBMCs) isolated from individuals with obesity relative to lean controls. We also investigated if impairments to PBMC metabolism were reversible upon short-term weight loss following bariatric surgery. RESULTS: Obesity was associated with systemic inflammation and poor inflammation resolution. Unstimulated PBMCs from participants with obesity had lower oxidative metabolism and adenosine triphosphate (ATP) production compared to PBMCs from lean controls. PBMC secretome analyses showed that ex vivo stimulation with A/Cal/7/2009 H1N1 influenza led to a notable increase in IL-6 with obesity. Short-term weight loss via bariatric surgery improved biomarkers of systemic metabolism but did not improve markers of inflammation resolution, PBMC metabolism, or the PBMC secretome. CONCLUSIONS: These results show that obesity drives a signature of impaired PBMC metabolism, which may be due to persistent inflammation. PBMC metabolism was not reversed after short-term weight loss despite improvements in measures of systemic metabolism.

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Published In

J Infect Dis

DOI

EISSN

1537-6613

Publication Date

December 28, 2022

Volume

227

Issue

1

Start / End Page

92 / 102

Location

United States

Related Subject Headings

  • Weight Loss
  • Obesity
  • Microbiology
  • Leukocytes, Mononuclear
  • Influenza, Human
  • Influenza A Virus, H1N1 Subtype
  • Inflammation
  • Humans
  • Bariatric Surgery
  • Adult
 

Citation

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Chicago
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Green, W. D., Alwarawrah, Y., Al-Shaer, A. E., Shi, Q., Armstrong, M., Manke, J., … Shaikh, S. R. (2022). Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery. J Infect Dis, 227(1), 92–102. https://doi.org/10.1093/infdis/jiac345
Green, William D., Yazan Alwarawrah, Abrar E. Al-Shaer, Qing Shi, Michael Armstrong, Jonathan Manke, Nichole Reisdorph, et al. “Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery.J Infect Dis 227, no. 1 (December 28, 2022): 92–102. https://doi.org/10.1093/infdis/jiac345.
Green WD, Alwarawrah Y, Al-Shaer AE, Shi Q, Armstrong M, Manke J, et al. Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery. J Infect Dis. 2022 Dec 28;227(1):92–102.
Green, William D., et al. “Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery.J Infect Dis, vol. 227, no. 1, Dec. 2022, pp. 92–102. Pubmed, doi:10.1093/infdis/jiac345.
Green WD, Alwarawrah Y, Al-Shaer AE, Shi Q, Armstrong M, Manke J, Reisdorph N, Farrell TM, Hursting SD, MacIver NJ, Beck MA, Shaikh SR. Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery. J Infect Dis. 2022 Dec 28;227(1):92–102.
Journal cover image

Published In

J Infect Dis

DOI

EISSN

1537-6613

Publication Date

December 28, 2022

Volume

227

Issue

1

Start / End Page

92 / 102

Location

United States

Related Subject Headings

  • Weight Loss
  • Obesity
  • Microbiology
  • Leukocytes, Mononuclear
  • Influenza, Human
  • Influenza A Virus, H1N1 Subtype
  • Inflammation
  • Humans
  • Bariatric Surgery
  • Adult