Detection of m6A in single cultured cells using scDART-seq.

Journal Article (Journal Article)

Most techniques for mapping m6A-methylated RNAs transcriptome-wide require large amounts of RNA and have been limited to bulk cells and tissues. Here, we provide a detailed protocol for the identification of m6A sites in single HEK293T cells using single-cell DART-seq (scDART-seq). The protocol details how to generate cell lines with inducible expression of the APOBEC1-YTH transgene and the use of important controls for minimizing false positives. We also describe the bioinformatic analysis to identify m6A sites. For complete details on the use and execution of this protocol, please refer to Tegowski et al. (2022).

Full Text

Duke Authors

Cited Authors

  • Tegowski, M; Meyer, KD

Published Date

  • September 16, 2022

Published In

Volume / Issue

  • 3 / 3

Start / End Page

  • 101646 -

PubMed ID

  • 36042888

Pubmed Central ID

  • PMC9420395

Electronic International Standard Serial Number (EISSN)

  • 2666-1667

Digital Object Identifier (DOI)

  • 10.1016/j.xpro.2022.101646

Language

  • eng

Conference Location

  • United States