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Formulation of diblock polymeric nanoparticles through nanoprecipitation technique.

Publication ,  Journal Article
Karve, S; Werner, ME; Cummings, ND; Sukumar, R; Wang, EC; Zhang, Y-A; Wang, AZ
Published in: J Vis Exp
September 20, 2011

Nanotechnology is a relatively new branch of science that involves harnessing the unique properties of particles that are nanometers in scale (nanoparticles). Nanoparticles can be engineered in a precise fashion where their size, composition and surface chemistry can be carefully controlled. This enables unprecedented freedom to modify some of the fundamental properties of their cargo, such as solubility, diffusivity, biodistribution, release characteristics and immunogenicity. Since their inception, nanoparticles have been utilized in many areas of science and medicine, including drug delivery, imaging, and cell biology(1-4). However, it has not been fully utilized outside of "nanotechnology laboratories" due to perceived technical barrier. In this article, we describe a simple method to synthesize a polymer based nanoparticle platform that has a wide range of potential applications. The first step is to synthesize a diblock co-polymer that has both a hydrophobic domain and hydrophilic domain. Using PLGA and PEG as model polymers, we described a conjugation reaction using EDC/NHS chemistry(5) (Fig 1). We also discuss the polymer purification process. The synthesized diblock co-polymer can self-assemble into nanoparticles in the nanoprecipitation process through hydrophobic-hydrophilic interactions. The described polymer nanoparticle is very versatile. The hydrophobic core of the nanoparticle can be utilized to carry poorly soluble drugs for drug delivery experiments6. Furthermore, the nanoparticles can overcome the problem of toxic solvents for poorly soluble molecular biology reagents, such as wortmannin, which requires a solvent like DMSO. However, DMSO can be toxic to cells and interfere with the experiment. These poorly soluble drugs and reagents can be effectively delivered using polymer nanoparticles with minimal toxicity. Polymer nanoparticles can also be loaded with fluorescent dye and utilized for intracellular trafficking studies. Lastly, these polymer nanoparticles can be conjugated to targeting ligands through surface PEG. Such targeted nanoparticles can be utilized to label specific epitopes on or in cells(7-10).

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Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

September 20, 2011

Issue

55

Location

United States

Related Subject Headings

  • Polymers
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Polyglactin 910
  • Polyethylene Glycols
  • Nanotechnology
  • Nanoparticles
  • Lactic Acid
  • Hydrophobic and Hydrophilic Interactions
  • 3101 Biochemistry and cell biology
 

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Karve, S., Werner, M. E., Cummings, N. D., Sukumar, R., Wang, E. C., Zhang, Y.-A., & Wang, A. Z. (2011). Formulation of diblock polymeric nanoparticles through nanoprecipitation technique. J Vis Exp, (55). https://doi.org/10.3791/3398
Karve, Shrirang, Michael E. Werner, Natalie D. Cummings, Rohit Sukumar, Edina C. Wang, Ying-Ao Zhang, and Andrew Z. Wang. “Formulation of diblock polymeric nanoparticles through nanoprecipitation technique.J Vis Exp, no. 55 (September 20, 2011). https://doi.org/10.3791/3398.
Karve S, Werner ME, Cummings ND, Sukumar R, Wang EC, Zhang Y-A, et al. Formulation of diblock polymeric nanoparticles through nanoprecipitation technique. J Vis Exp. 2011 Sep 20;(55).
Karve, Shrirang, et al. “Formulation of diblock polymeric nanoparticles through nanoprecipitation technique.J Vis Exp, no. 55, Sept. 2011. Pubmed, doi:10.3791/3398.
Karve S, Werner ME, Cummings ND, Sukumar R, Wang EC, Zhang Y-A, Wang AZ. Formulation of diblock polymeric nanoparticles through nanoprecipitation technique. J Vis Exp. 2011 Sep 20;(55).

Published In

J Vis Exp

DOI

EISSN

1940-087X

Publication Date

September 20, 2011

Issue

55

Location

United States

Related Subject Headings

  • Polymers
  • Polylactic Acid-Polyglycolic Acid Copolymer
  • Polyglycolic Acid
  • Polyglactin 910
  • Polyethylene Glycols
  • Nanotechnology
  • Nanoparticles
  • Lactic Acid
  • Hydrophobic and Hydrophilic Interactions
  • 3101 Biochemistry and cell biology