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Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy.

Publication ,  Journal Article
Pla, MM; Evans, A; Lezberg, P; Bowles, DE
Published in: Methods Mol Biol
2022

Recent advances in ex vivo perfusion have enabled an extended preservation time for solid organs prior to transplantation allowing for possible resuscitation of the donor organ during the preservation period. Opportunities to provide viral vector-mediated gene therapy to the entire cardiac graft during this extended preservation period may lead to improvements in cardiac transplantation outcomes. Here we describe how to achieve successful gene delivery using viral vectors to an entire cardiac graft by normothermic, ex vivo perfusion. This protocol has been confirmed with the most highly utilized viral vector types in gene therapy clinical studies (adenoviral [Ad] and adeno-associated viral vector [AAV]).

Duke Scholars

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2022

Volume

2573

Start / End Page

249 / 259

Location

United States

Related Subject Headings

  • Tissue Donors
  • Perfusion
  • Humans
  • Heart Transplantation
  • Genetic Vectors
  • Genetic Therapy
  • Developmental Biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pla, M. M., Evans, A., Lezberg, P., & Bowles, D. E. (2022). Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy. Methods Mol Biol, 2573, 249–259. https://doi.org/10.1007/978-1-0716-2707-5_19
Pla, Michelle Mendiola, Amy Evans, Paul Lezberg, and Dawn E. Bowles. “Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy.Methods Mol Biol 2573 (2022): 249–59. https://doi.org/10.1007/978-1-0716-2707-5_19.
Pla MM, Evans A, Lezberg P, Bowles DE. Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy. Methods Mol Biol. 2022;2573:249–59.
Pla, Michelle Mendiola, et al. “Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy.Methods Mol Biol, vol. 2573, 2022, pp. 249–59. Pubmed, doi:10.1007/978-1-0716-2707-5_19.
Pla MM, Evans A, Lezberg P, Bowles DE. Ex Vivo Delivery of Viral Vectors by Organ Perfusion for Cardiac Transplantation Gene Therapy. Methods Mol Biol. 2022;2573:249–259.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2022

Volume

2573

Start / End Page

249 / 259

Location

United States

Related Subject Headings

  • Tissue Donors
  • Perfusion
  • Humans
  • Heart Transplantation
  • Genetic Vectors
  • Genetic Therapy
  • Developmental Biology
  • 0601 Biochemistry and Cell Biology
  • 0399 Other Chemical Sciences