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Standardization of the collection of exhaled breath condensate and exhaled breath aerosol using a feedback regulated sampling device.

Publication ,  Journal Article
Winters, BR; Pleil, JD; Angrish, MM; Stiegel, MA; Risby, TH; Madden, MC
Published in: Journal of breath research
November 2017

Exhaled breath condensate (EBC) and associated exhaled breath aerosols (EBA) are valuable non-invasive biological media used for the quantification of biomarkers. EBC contains exhaled water vapor, soluble gas-phase (polar) organic compounds, ionic species, plus other species including semi- and non-volatile organic compounds, proteins, cell fragments, DNA, dissolved inorganic compounds, ions, and microbiota (bacteria and viruses) dissolved in the co-collected EBA. EBC is collected from subjects who breathe 'normally' through a chilled tube assembly for approximately 10 min and is then harvested into small vials for analysis. Aerosol filters without the chilled tube assembly are also used to separately collect EBA. Unlike typical gas-phase breath samples used for environmental and clinical applications, the constituents of EBC and EBA are not easily characterized by total volume or carbon dioxide (CO2) concentration, because the gas-phase is vented. Furthermore, EBC and associated EBA are greatly affected by breathing protocol, more specifically, depth of inhalation and expelled breath velocity. We have tested a new instrument developed by Loccioni Gruppa Humancare (Ancona, Italy) for implementation of EBC collection from human subjects to assess EBC collection parameters. The instrument is the first EBC collection device that provides instantaneous visual feedback to the subjects to control breathing patterns. In this report we describe the operation of the instrument, and present an overview of performance and analytical applications.

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Published In

Journal of breath research

DOI

EISSN

1752-7163

ISSN

1752-7155

Publication Date

November 2017

Volume

11

Issue

4

Start / End Page

047107

Related Subject Headings

  • Reference Standards
  • Hydrogen-Ion Concentration
  • Humans
  • Feedback
  • Exhalation
  • Breath Tests
  • Biomarkers
  • Aerosols
  • Adult
  • 4003 Biomedical engineering
 

Citation

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Winters, B. R., Pleil, J. D., Angrish, M. M., Stiegel, M. A., Risby, T. H., & Madden, M. C. (2017). Standardization of the collection of exhaled breath condensate and exhaled breath aerosol using a feedback regulated sampling device. Journal of Breath Research, 11(4), 047107. https://doi.org/10.1088/1752-7163/aa8bbc
Winters, Brett R., Joachim D. Pleil, Michelle M. Angrish, Matthew A. Stiegel, Terence H. Risby, and Michael C. Madden. “Standardization of the collection of exhaled breath condensate and exhaled breath aerosol using a feedback regulated sampling device.Journal of Breath Research 11, no. 4 (November 2017): 047107. https://doi.org/10.1088/1752-7163/aa8bbc.
Winters BR, Pleil JD, Angrish MM, Stiegel MA, Risby TH, Madden MC. Standardization of the collection of exhaled breath condensate and exhaled breath aerosol using a feedback regulated sampling device. Journal of breath research. 2017 Nov;11(4):047107.
Winters, Brett R., et al. “Standardization of the collection of exhaled breath condensate and exhaled breath aerosol using a feedback regulated sampling device.Journal of Breath Research, vol. 11, no. 4, Nov. 2017, p. 047107. Epmc, doi:10.1088/1752-7163/aa8bbc.
Winters BR, Pleil JD, Angrish MM, Stiegel MA, Risby TH, Madden MC. Standardization of the collection of exhaled breath condensate and exhaled breath aerosol using a feedback regulated sampling device. Journal of breath research. 2017 Nov;11(4):047107.
Journal cover image

Published In

Journal of breath research

DOI

EISSN

1752-7163

ISSN

1752-7155

Publication Date

November 2017

Volume

11

Issue

4

Start / End Page

047107

Related Subject Headings

  • Reference Standards
  • Hydrogen-Ion Concentration
  • Humans
  • Feedback
  • Exhalation
  • Breath Tests
  • Biomarkers
  • Aerosols
  • Adult
  • 4003 Biomedical engineering