Optimization of an Antibody Microarray Printing Process Using a Designed Experiment.

Journal Article (Journal Article)

Antibody microarrays have proven useful in immunoassay-based point-of-care diagnostics for infectious diseases. Noncontact piezoelectric inkjet printing has advantages to print antibody microarrays on nitrocellulose substrates for this application due to its compatibility with sensitive solutions and substrates, simple droplet control, and potential for high-capacity printing. However, there remain real-world challenges in printing such microarrays, which motivated this study. The effects of three concentrations of capture antibody (cAb) reagents and nozzle hydrostatic pressures were chosen to investigate three responses: the number of printed membrane disks, dispensing performance, and microarray quality. Printing conditions were found to be most ideal with 5 mg/mL cAb and a nozzle hydrostatic pressure near zero, which produced 130 membrane disks in a single print versus the 10 membrane disks per print before optimization. These results serve to inform efficient printing of antibody microarrays on nitrocellulose membranes for rapid immunoassay-based detection of infectious diseases and beyond.

Full Text

Duke Authors

Cited Authors

  • Summers, AJ; Devadhasan, JP; Gu, J; Montgomery, DC; Fischer, B; Gates-Hollingsworth, MA; Pflughoeft, KJ; Vo-Dinh, T; AuCoin, DP; Zenhausern, F

Published Date

  • September 2022

Published In

Volume / Issue

  • 7 / 36

Start / End Page

  • 32262 - 32271

PubMed ID

  • 36120062

Pubmed Central ID

  • PMC9476517

Electronic International Standard Serial Number (EISSN)

  • 2470-1343

International Standard Serial Number (ISSN)

  • 2470-1343

Digital Object Identifier (DOI)

  • 10.1021/acsomega.2c03595


  • eng