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Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.

Publication ,  Journal Article
Zhang, R; Li, LS; Rao, B; Rong, H; Sun, M-Y; Yao, J; Chen, R; Zhou, Q; Mennerick, S; Raman, B; Wang, LV
Published in: Journal of biomedical optics
September 2022

Optical imaging of responses in fluorescently labeled neurons has progressed significantly in recent years. However, there is still a need to monitor neural activities at divergent spatial scales and at depths beyond the optical diffusion limit.To meet these needs, we aim to develop multiscale photoacoustic tomography (PAT) to image neural activities across spatial scales with a genetically encoded calcium indicator GCaMP.First, using photoacoustic microscopy, we show that depth-resolved GCaMP signals can be monitored in vivo from a fly brain in response to odor stimulation without depth scanning and even with the cuticle intact. In vivo monitoring of GCaMP signals was also demonstrated in mouse brains. Next, using photoacoustic computed tomography, we imaged neural responses of a mouse brain slice at depths beyond the optical diffusion limit.We provide the first unambiguous demonstration that multiscale PAT can be used to record neural activities in transgenic flies and mice with select neurons expressing GCaMP.Our results indicate that the combination of multiscale PAT and fluorescent neural activity indicators provides a methodology for imaging targeted neurons at various scales.

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Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

September 2022

Volume

27

Issue

9

Related Subject Headings

  • Tomography, X-Ray Computed
  • Optics
  • Optical Imaging
  • Neurons
  • Mice
  • Calcium
  • Brain
  • Animals
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering
 

Citation

APA
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ICMJE
MLA
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Zhang, R., Li, L. S., Rao, B., Rong, H., Sun, M.-Y., Yao, J., … Wang, L. V. (2022). Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators. Journal of Biomedical Optics, 27(9). https://doi.org/10.1117/1.jbo.27.9.096004
Zhang, Ruiying, Lei S. Li, Bin Rao, Haoyang Rong, Min-Yu Sun, Junjie Yao, Ruimin Chen, et al. “Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.Journal of Biomedical Optics 27, no. 9 (September 2022). https://doi.org/10.1117/1.jbo.27.9.096004.
Zhang R, Li LS, Rao B, Rong H, Sun M-Y, Yao J, et al. Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators. Journal of biomedical optics. 2022 Sep;27(9).
Zhang, Ruiying, et al. “Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators.Journal of Biomedical Optics, vol. 27, no. 9, Sept. 2022. Epmc, doi:10.1117/1.jbo.27.9.096004.
Zhang R, Li LS, Rao B, Rong H, Sun M-Y, Yao J, Chen R, Zhou Q, Mennerick S, Raman B, Wang LV. Multiscale photoacoustic tomography of neural activities with GCaMP calcium indicators. Journal of biomedical optics. 2022 Sep;27(9).

Published In

Journal of biomedical optics

DOI

EISSN

1560-2281

ISSN

1083-3668

Publication Date

September 2022

Volume

27

Issue

9

Related Subject Headings

  • Tomography, X-Ray Computed
  • Optics
  • Optical Imaging
  • Neurons
  • Mice
  • Calcium
  • Brain
  • Animals
  • 5102 Atomic, molecular and optical physics
  • 4003 Biomedical engineering