Self-assembling peptide nanofiber HIV vaccine elicits robust vaccine-induced antibody functions and modulates Fc glycosylation.

Journal Article (Journal Article)

To develop vaccines for certain key global pathogens such as HIV, it is crucial to elicit both neutralizing and non-neutralizing Fc-mediated effector antibody functions. Clinical evidence indicates that non-neutralizing antibody functions including antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP) contribute to protection against several pathogens. In this study, we demonstrated that conjugation of HIV Envelope (Env) antigen gp120 to a self-assembling nanofiber material named Q11 induced antibodies with higher breadth and functionality when compared to soluble gp120. Immunization with Q11-conjugated gp120 vaccine (gp120-Q11) demonstrated higher tier 1 neutralization, ADCP, and ADCC as compared to soluble gp120. Moreover, Q11 conjugation altered the Fc N-glycosylation profile of antigen-specific antibodies, leading to a phenotype associated with increased ADCC in animals immunized with gp120-Q11. Thus, this nanomaterial vaccine strategy can enhance non-neutralizing antibody functions possibly through modulation of immunoglobulin G Fc N-glycosylation.

Full Text

Duke Authors

Cited Authors

  • Chen, J-L; Fries, CN; Berendam, SJ; Rodgers, NS; Roe, EF; Wu, Y; Li, SH; Jain, R; Watts, B; Eudailey, J; Barfield, R; Chan, C; Moody, MA; Saunders, KO; Pollara, J; Permar, SR; Collier, JH; Fouda, GG

Published Date

  • September 23, 2022

Published In

Volume / Issue

  • 8 / 38

Start / End Page

  • eabq0273 -

PubMed ID

  • 36149967

Pubmed Central ID

  • PMC9506727

Electronic International Standard Serial Number (EISSN)

  • 2375-2548

Digital Object Identifier (DOI)

  • 10.1126/sciadv.abq0273


  • eng

Conference Location

  • United States