A controlled-release strategy for the generation of cross-linked hydrogel microstructures.

Journal Article (Journal Article)

Microscale hydrogels of controlled sizes and shapes are useful for cell-based screening, in vitro diagnostics, tissue engineering, and drug delivery. However, the rapid cross-linking of many chemically and pH cross-linkable hydrogel materials prevents the application of existing micromolding techniques. In this work we present a method for fabricating micromolded calcium alginate and chitosan structures through controlled release of the gelling agent from a hydrogel mold. Replica molding was employed to generate patterned membranes, whereas microtransfer molding was used to produce microparticles of controlled shapes. To explore the viability of this technique for producing complex tissue engineering micro-architectures, this approach was used to generate cell-laden size- and shape-controlled 3D microgels as well as composite hydrogels with well-defined spatially segregated regions. In addition, shape-controlled microstructures that can exhibit differential release properties were loaded with macromolecules to verify the potential of this approach for drug delivery applications.

Full Text

Duke Authors

Cited Authors

  • Franzesi, GT; Ni, B; Ling, Y; Khademhosseini, A

Published Date

  • November 2006

Published In

Volume / Issue

  • 128 / 47

Start / End Page

  • 15064 - 15065

PubMed ID

  • 17117838

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja065867x


  • eng