Selective Autophagy of BES1 Mediated by DSK2 Balances Plant Growth and Survival.

Journal Article (Journal Article)

Plants encounter a variety of stresses and must fine-tune their growth and stress-response programs to best suit their environment. BES1 functions as a master regulator in the brassinosteroid (BR) pathway that promotes plant growth. Here, we show that BES1 interacts with the ubiquitin receptor protein DSK2 and is targeted to the autophagy pathway during stress via the interaction of DSK2 with ATG8, a ubiquitin-like protein directing autophagosome formation and cargo recruitment. Additionally, DSK2 is phosphorylated by the GSK3-like kinase BIN2, a negative regulator in the BR pathway. BIN2 phosphorylation of DSK2 flanking its ATG8 interacting motifs (AIMs) promotes DSK2-ATG8 interaction, thereby targeting BES1 for degradation. Accordingly, loss-of-function dsk2 mutants accumulate BES1, have altered global gene expression profiles, and have compromised stress responses. Our results thus reveal that plants coordinate growth and stress responses by integrating BR and autophagy pathways and identify the molecular basis of this crosstalk.

Full Text

Duke Authors

Cited Authors

  • Nolan, TM; Brennan, B; Yang, M; Chen, J; Zhang, M; Li, Z; Wang, X; Bassham, DC; Walley, J; Yin, Y

Published Date

  • April 2017

Published In

Volume / Issue

  • 41 / 1

Start / End Page

  • 33 - 46.e7

PubMed ID

  • 28399398

Pubmed Central ID

  • PMC5720862

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2017.03.013

Language

  • eng