Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.
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Nolan, T; Liu, S; Guo, H; Li, L; Schnable, P; Yin, Y
January 2017
Brassinosteroids (BRs) play important roles in many growth and developmental processes. BRs signal to regulate BR-INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1 (BES1) and BRASSINAZOLE-RESISTANT1 (BZR1) transcription factors (TFs), which, in turn, regulate several hundreds of transcription factors (termed BES1/BZR1-targeted TFs or BTFs) and thousands of genes to mediate various BR responses. Chromatin Immunoprecipitation followed by high-throughput sequencing (ChIP-seq) with BES1/BZR1 and BTFs is an important approach to identify BR target genes. In combination with RNA-sequencing experiments, these genomic methods have become powerful tools to detect BR target genes and reveal transcriptional networks underlying BR-regulated processes.
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DOI
Publication Date
January 2017
Volume
1564
Start / End Page
63 / 79
Related Subject Headings
- Steroids, Heterocyclic
- Signal Transduction
- Seedlings
- Plant Growth Regulators
- Nuclear Proteins
- High-Throughput Nucleotide Sequencing
- Gene Expression Regulation, Plant
- Gene Expression Regulation, Developmental
- Developmental Biology
- DNA-Binding Proteins
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Nolan, T., Liu, S., Guo, H., Li, L., Schnable, P., & Yin, Y. (2017). Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing. (Vol. 1564, pp. 63–79). https://doi.org/10.1007/978-1-4939-6813-8_7
Nolan, Trevor, Sanzhen Liu, Hongqing Guo, Lei Li, Patrick Schnable, and Yanhai Yin. “Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing.,” 1564:63–79, 2017. https://doi.org/10.1007/978-1-4939-6813-8_7.
Nolan T, Liu S, Guo H, Li L, Schnable P, Yin Y. Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing. In 2017. p. 63–79.
Nolan, Trevor, et al. Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing. Vol. 1564, 2017, pp. 63–79. Epmc, doi:10.1007/978-1-4939-6813-8_7.
Nolan T, Liu S, Guo H, Li L, Schnable P, Yin Y. Identification of Brassinosteroid Target Genes by Chromatin Immunoprecipitation Followed by High-Throughput Sequencing (ChIP-seq) and RNA-Sequencing. 2017. p. 63–79.
DOI
Publication Date
January 2017
Volume
1564
Start / End Page
63 / 79
Related Subject Headings
- Steroids, Heterocyclic
- Signal Transduction
- Seedlings
- Plant Growth Regulators
- Nuclear Proteins
- High-Throughput Nucleotide Sequencing
- Gene Expression Regulation, Plant
- Gene Expression Regulation, Developmental
- Developmental Biology
- DNA-Binding Proteins