Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum.
Publication
, Journal Article
Cook, CE; Twine, ME; Myers, M; Amerson, E; Kepler, JA; Taylor, GF
Published in: Res Commun Chem Pathol Pharmacol
March 1976
Antisera to theophylline (T) have been obtained by immunizing rabbits with a conjugate of 8-(3-carboxypropyl)-1,3-dimethylxanthine and bovine serum albumin. Comparison of 50% displacement values indicated good selectivity for T vs. a number of other xanthine derivatives. An analytical procedure using this antiserum can measure 200 pg of T and direct analysis of 0.1 mug/ml in plasma or 0.02 mug/ml in saliva is feasible.
Duke Scholars
Published In
Res Commun Chem Pathol Pharmacol
ISSN
0034-5164
Publication Date
March 1976
Volume
13
Issue
3
Start / End Page
497 / 505
Location
United States
Related Subject Headings
- Theophylline
- Theobromine
- Serum Albumin, Bovine
- Saliva
- Radioimmunoassay
- Rabbits
- Microchemistry
- Male
- Immune Sera
- Female
Citation
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ICMJE
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Cook, C. E., Twine, M. E., Myers, M., Amerson, E., Kepler, J. A., & Taylor, G. F. (1976). Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum. Res Commun Chem Pathol Pharmacol, 13(3), 497–505.
Cook, C. E., M. E. Twine, M. Myers, E. Amerson, J. A. Kepler, and G. F. Taylor. “Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum.” Res Commun Chem Pathol Pharmacol 13, no. 3 (March 1976): 497–505.
Cook CE, Twine ME, Myers M, Amerson E, Kepler JA, Taylor GF. Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum. Res Commun Chem Pathol Pharmacol. 1976 Mar;13(3):497–505.
Cook, C. E., et al. “Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum.” Res Commun Chem Pathol Pharmacol, vol. 13, no. 3, Mar. 1976, pp. 497–505.
Cook CE, Twine ME, Myers M, Amerson E, Kepler JA, Taylor GF. Theophylline radioimmunoassay: synthesis of antigen and characterization of antiserum. Res Commun Chem Pathol Pharmacol. 1976 Mar;13(3):497–505.
Published In
Res Commun Chem Pathol Pharmacol
ISSN
0034-5164
Publication Date
March 1976
Volume
13
Issue
3
Start / End Page
497 / 505
Location
United States
Related Subject Headings
- Theophylline
- Theobromine
- Serum Albumin, Bovine
- Saliva
- Radioimmunoassay
- Rabbits
- Microchemistry
- Male
- Immune Sera
- Female