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Confirmation of cannabis use. II. Determination of tetrahydrocannabinol metabolites in urine and plasma by HPLC with ECD.

Publication ,  Journal Article
Nakahara, Y; Sekine, H; Cook, CE
Published in: J Anal Toxicol
1989

Simultaneous determination of delta 9-tetrahydrocannabinol (THC) and its major metabolites--11-nor-delta 9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) and 11-hydroxy-delta 9-tetrahydrocannabinol (11-OH THC)--in urine and plasma by HPLC with electrochemical detection (LC/EC) was studied. After biological fluids were hydrolyzed, samples were extracted with an automatic extractor and analyzed by LC/EC. HPLC was carried out with a reversed-phase silica C8 column and a mobile phase of acetonitrile/methanol/0.02N H2SO4 (35:15:50) at 1.8 mL/min. Coefficients of variation at 10-500 ng/mL of THC metabolites were 2.19-5.91%. The detection limit was under 0.5 ng/mL (S/N greater than 3). Time course of excretion of THC and THC-COOH in rabbit urine was studied. THC-COOH could be detected until 216 h after administration. By this method, urine samples of suspects arrested in Japan were tested.

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Published In

J Anal Toxicol

DOI

ISSN

0146-4760

Publication Date

1989

Volume

13

Issue

1

Start / End Page

22 / 24

Location

England

Related Subject Headings

  • Rabbits
  • Male
  • Humans
  • Electrochemistry
  • Dronabinol
  • Chromatography, High Pressure Liquid
  • Animals
  • Analytical Chemistry
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0301 Analytical Chemistry
 

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Nakahara, Y., Sekine, H., & Cook, C. E. (1989). Confirmation of cannabis use. II. Determination of tetrahydrocannabinol metabolites in urine and plasma by HPLC with ECD. J Anal Toxicol, 13(1), 22–24. https://doi.org/10.1093/jat/13.1.22
Nakahara, Y., H. Sekine, and C. E. Cook. “Confirmation of cannabis use. II. Determination of tetrahydrocannabinol metabolites in urine and plasma by HPLC with ECD.J Anal Toxicol 13, no. 1 (1989): 22–24. https://doi.org/10.1093/jat/13.1.22.
Nakahara, Y., et al. “Confirmation of cannabis use. II. Determination of tetrahydrocannabinol metabolites in urine and plasma by HPLC with ECD.J Anal Toxicol, vol. 13, no. 1, 1989, pp. 22–24. Pubmed, doi:10.1093/jat/13.1.22.
Journal cover image

Published In

J Anal Toxicol

DOI

ISSN

0146-4760

Publication Date

1989

Volume

13

Issue

1

Start / End Page

22 / 24

Location

England

Related Subject Headings

  • Rabbits
  • Male
  • Humans
  • Electrochemistry
  • Dronabinol
  • Chromatography, High Pressure Liquid
  • Animals
  • Analytical Chemistry
  • 1115 Pharmacology and Pharmaceutical Sciences
  • 0301 Analytical Chemistry