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Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation.

Publication ,  Journal Article
McCarthy, MK; Procario, MC; Wilke, CA; Moore, BB; Weinberg, JB
Published in: PLoS One
2015

Adenovirus infections are important complications of bone marrow transplantation (BMT). We demonstrate delayed clearance of mouse adenovirus type 1 (MAV-1) from lungs of mice following allogeneic BMT. Virus-induced prostaglandin E2 (PGE2) production was greater in BMT mice than in untransplanted controls, but BMT using PGE2-deficient donors or recipients failed to improve viral clearance, and treatment of untransplanted mice with the PGE2 analog misoprostol did not affect virus clearance. Lymphocyte recruitment to the lungs was not significantly affected by BMT. Intracellular cytokine staining of lung lymphocytes demonstrated impaired production of INF-γ and granzyme B by cells from BMT mice, and production of IFN-γ, IL-2, IL-4, and IL-17 following ex vivo stimulation was impaired in lymphocytes obtained from lungs of BMT mice. Viral clearance was not delayed in untransplanted INF-γ-deficient mice, suggesting that delayed viral clearance in BMT mice was not a direct consequence of impaired IFN-γ production. However, lung viral loads were higher in untransplanted CD8-deficient mice than in controls, suggesting that delayed MAV-1 clearance in BMT mice is due to defective CD8 T cell function. We did not detect significant induction of IFN-β expression in lungs of BMT mice or untransplanted controls, and viral clearance was not delayed in untransplanted type I IFN-unresponsive mice. We conclude that PGE2 overproduction in BMT mice is not directly responsible for delayed viral clearance. PGE2-independent effects on CD8 T cell function likely contribute to the inability of BMT mice to clear MAV-1 from the lungs.

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Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

9

Start / End Page

e0139235

Location

United States

Related Subject Headings

  • Viral Load
  • T-Lymphocytes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lung
  • General Science & Technology
  • Dinoprostone
 

Citation

APA
Chicago
ICMJE
MLA
NLM
McCarthy, M. K., Procario, M. C., Wilke, C. A., Moore, B. B., & Weinberg, J. B. (2015). Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation. PLoS One, 10(9), e0139235. https://doi.org/10.1371/journal.pone.0139235
McCarthy, Mary K., Megan C. Procario, Carol A. Wilke, Bethany B. Moore, and Jason B. Weinberg. “Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation.PLoS One 10, no. 9 (2015): e0139235. https://doi.org/10.1371/journal.pone.0139235.
McCarthy MK, Procario MC, Wilke CA, Moore BB, Weinberg JB. Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation. PLoS One. 2015;10(9):e0139235.
McCarthy, Mary K., et al. “Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation.PLoS One, vol. 10, no. 9, 2015, p. e0139235. Pubmed, doi:10.1371/journal.pone.0139235.
McCarthy MK, Procario MC, Wilke CA, Moore BB, Weinberg JB. Prostaglandin E2 Production and T Cell Function in Mouse Adenovirus Type 1 Infection following Allogeneic Bone Marrow Transplantation. PLoS One. 2015;10(9):e0139235.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2015

Volume

10

Issue

9

Start / End Page

e0139235

Location

United States

Related Subject Headings

  • Viral Load
  • T-Lymphocytes
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice, Inbred BALB C
  • Mice
  • Male
  • Lung
  • General Science & Technology
  • Dinoprostone