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Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells.

Publication ,  Journal Article
Wang, Z-B; Boisvert, E; Zhang, X; Guo, M; Fashoyin, A; Du, Z-W; Zhang, S-C; Li, X-J
Published in: Cereb Cortex
September 2011

The mechanisms by which transcription factors control stepwise lineage restriction during the specification of cortical neurons remain largely unknown. Here, we investigated the role of forebrain embryonic zinc finger like (Fezf2) in this process by generating Fezf2 knockdown and tetracycline-inducible Fezf2 overexpression mouse embryonic stem cell (mESC) lines. The overexpression of Fezf2 at early time points significantly increased the generation of rostral forebrain progenitors (Foxg1(+), Six3(+)) and inhibited the expression of transcription factors which are expressed by the midbrain and caudal diencephalon (En1(+), Irx(+)). This effect was partially achieved by the regulation of Wnt signaling during this critical early time window. The role of Fezf2 in regulating the rostrocaudal patterning was further confirmed by the significant decrease in the expression of Foxg1 and Six3 and the increase in the expression of En1 when Fezf2 was knocked down. In addition, Fezf2 overexpression at later time points had little effect on the expression of Foxg1 and Six3. Instead, Fezf2 promotes the generation of dorsal telencephalic progenitors and deep-layer cortical neurons at later stages. Collectively, our data suggest that Fezf2 controls the specification of telencephalic progenitors from mESCs through differentially regulating the expression of rostrocaudal and dorsoventral patterning genes.

Duke Scholars

Published In

Cereb Cortex

DOI

EISSN

1460-2199

Publication Date

September 2011

Volume

21

Issue

9

Start / End Page

2177 / 2186

Location

United States

Related Subject Headings

  • Wnt Signaling Pathway
  • Transfection
  • Transcription Factors
  • Telencephalon
  • Reverse Transcriptase Polymerase Chain Reaction
  • Prosencephalon
  • Neural Stem Cells
  • Nerve Tissue Proteins
  • Mice
  • Lentivirus
 

Citation

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Wang, Z.-B., Boisvert, E., Zhang, X., Guo, M., Fashoyin, A., Du, Z.-W., … Li, X.-J. (2011). Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells. Cereb Cortex, 21(9), 2177–2186. https://doi.org/10.1093/cercor/bhr006
Wang, Zhi-Bo, Erin Boisvert, Xiaoqing Zhang, Min Guo, Adedayo Fashoyin, Zhong-Wei Du, Su-Chun Zhang, and Xue-Jun Li. “Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells.Cereb Cortex 21, no. 9 (September 2011): 2177–86. https://doi.org/10.1093/cercor/bhr006.
Wang Z-B, Boisvert E, Zhang X, Guo M, Fashoyin A, Du Z-W, et al. Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells. Cereb Cortex. 2011 Sep;21(9):2177–86.
Wang, Zhi-Bo, et al. “Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells.Cereb Cortex, vol. 21, no. 9, Sept. 2011, pp. 2177–86. Pubmed, doi:10.1093/cercor/bhr006.
Wang Z-B, Boisvert E, Zhang X, Guo M, Fashoyin A, Du Z-W, Zhang S-C, Li X-J. Fezf2 regulates telencephalic precursor differentiation from mouse embryonic stem cells. Cereb Cortex. 2011 Sep;21(9):2177–2186.
Journal cover image

Published In

Cereb Cortex

DOI

EISSN

1460-2199

Publication Date

September 2011

Volume

21

Issue

9

Start / End Page

2177 / 2186

Location

United States

Related Subject Headings

  • Wnt Signaling Pathway
  • Transfection
  • Transcription Factors
  • Telencephalon
  • Reverse Transcriptase Polymerase Chain Reaction
  • Prosencephalon
  • Neural Stem Cells
  • Nerve Tissue Proteins
  • Mice
  • Lentivirus