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Efficacy of BET bromodomain inhibition in Kras-mutant non-small cell lung cancer.

Publication ,  Journal Article
Shimamura, T; Chen, Z; Soucheray, M; Carretero, J; Kikuchi, E; Tchaicha, JH; Gao, Y; Cheng, KA; Cohoon, TJ; Qi, J; Akbay, E; Kimmelman, AC ...
Published in: Clin Cancer Res
November 15, 2013

PURPOSE: Amplification of MYC is one of the most common genetic alterations in lung cancer, contributing to a myriad of phenotypes associated with growth, invasion, and drug resistance. Murine genetics has established both the centrality of somatic alterations of Kras in lung cancer, as well as the dependency of mutant Kras tumors on MYC function. Unfortunately, drug-like small-molecule inhibitors of KRAS and MYC have yet to be realized. The recent discovery, in hematologic malignancies, that bromodomain and extra-terminal (BET) bromodomain inhibition impairs MYC expression and MYC transcriptional function established the rationale of targeting KRAS-driven non-small cell lung cancer (NSCLC) with BET inhibition. EXPERIMENTAL DESIGN: We performed functional assays to evaluate the effects of JQ1 in genetically defined NSCLC cell lines harboring KRAS and/or LKB1 mutations. Furthermore, we evaluated JQ1 in transgenic mouse lung cancer models expressing mutant kras or concurrent mutant kras and lkb1. Effects of bromodomain inhibition on transcriptional pathways were explored and validated by expression analysis. RESULTS: Although JQ1 is broadly active in NSCLC cells, activity of JQ1 in mutant KRAS NSCLC is abrogated by concurrent alteration or genetic knockdown of LKB1. In sensitive NSCLC models, JQ1 treatment results in the coordinate downregulation of the MYC-dependent transcriptional program. We found that JQ1 treatment produces significant tumor regression in mutant kras mice. As predicted, tumors from mutant kras and lkb1 mice did not respond to JQ1. CONCLUSION: Bromodomain inhibition comprises a promising therapeutic strategy for KRAS-mutant NSCLC with wild-type LKB1, via inhibition of MYC function. Clinical studies of BET bromodomain inhibitors in aggressive NSCLC will be actively pursued. Clin Cancer Res; 19(22); 6183-92. ©2013 AACR.

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Published In

Clin Cancer Res

DOI

EISSN

1557-3265

Publication Date

November 15, 2013

Volume

19

Issue

22

Start / End Page

6183 / 6192

Location

United States

Related Subject Headings

  • Triazoles
  • Transcription Factors
  • Signal Transduction
  • RNA, Small Interfering
  • RNA Interference
  • Proto-Oncogene Proteins p21(ras)
  • Proto-Oncogene Proteins c-myc
  • Protein Serine-Threonine Kinases
  • Oncology & Carcinogenesis
  • Nuclear Proteins
 

Citation

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Shimamura, T., Chen, Z., Soucheray, M., Carretero, J., Kikuchi, E., Tchaicha, J. H., … Wong, K.-K. (2013). Efficacy of BET bromodomain inhibition in Kras-mutant non-small cell lung cancer. Clin Cancer Res, 19(22), 6183–6192. https://doi.org/10.1158/1078-0432.CCR-12-3904
Shimamura, Takeshi, Zhao Chen, Margaret Soucheray, Julian Carretero, Eiki Kikuchi, Jeremy H. Tchaicha, Yandi Gao, et al. “Efficacy of BET bromodomain inhibition in Kras-mutant non-small cell lung cancer.Clin Cancer Res 19, no. 22 (November 15, 2013): 6183–92. https://doi.org/10.1158/1078-0432.CCR-12-3904.
Shimamura T, Chen Z, Soucheray M, Carretero J, Kikuchi E, Tchaicha JH, et al. Efficacy of BET bromodomain inhibition in Kras-mutant non-small cell lung cancer. Clin Cancer Res. 2013 Nov 15;19(22):6183–92.
Shimamura, Takeshi, et al. “Efficacy of BET bromodomain inhibition in Kras-mutant non-small cell lung cancer.Clin Cancer Res, vol. 19, no. 22, Nov. 2013, pp. 6183–92. Pubmed, doi:10.1158/1078-0432.CCR-12-3904.
Shimamura T, Chen Z, Soucheray M, Carretero J, Kikuchi E, Tchaicha JH, Gao Y, Cheng KA, Cohoon TJ, Qi J, Akbay E, Kimmelman AC, Kung AL, Bradner JE, Wong K-K. Efficacy of BET bromodomain inhibition in Kras-mutant non-small cell lung cancer. Clin Cancer Res. 2013 Nov 15;19(22):6183–6192.

Published In

Clin Cancer Res

DOI

EISSN

1557-3265

Publication Date

November 15, 2013

Volume

19

Issue

22

Start / End Page

6183 / 6192

Location

United States

Related Subject Headings

  • Triazoles
  • Transcription Factors
  • Signal Transduction
  • RNA, Small Interfering
  • RNA Interference
  • Proto-Oncogene Proteins p21(ras)
  • Proto-Oncogene Proteins c-myc
  • Protein Serine-Threonine Kinases
  • Oncology & Carcinogenesis
  • Nuclear Proteins