Ultrarapid detection of pathogenic bacteria using a 3D immunomagnetic flow assay.

Journal Article (Journal Article)

We developed a novel 3D immunomagnetic flow assay for the rapid detection of pathogenic bacteria in a large-volume food sample. Antibody-functionalized magnetic nanoparticle clusters (AbMNCs) were magnetically immobilized on the surfaces of a 3D-printed cylindrical microchannel. The injection of a Salmonella-spiked sample solution into the microchannel produced instant binding between the AbMNCs and the Salmonella bacteria due to their efficient collisions. Nearly perfect capture of the AbMNCs and AbMNCs-Salmonella complexes was achieved under a high flow rate by stacking permanent magnets with spacers inside the cylindrical separator to maximize the magnetic force. The concentration of the bacteria in solution was determined using ATP luminescence measurements. The detection limit was better than 10 cfu/mL, and the overall assay time, including the binding, rinsing, and detection steps for a 10 mL sample took less than 3 min. To our knowledge, the 3D immunomagnetic flow assay described here provides the fastest high-sensitivity, high-capacity method for the detection of pathogenic bacteria.

Full Text

Duke Authors

Cited Authors

  • Lee, W; Kwon, D; Chung, B; Jung, GY; Au, A; Folch, A; Jeon, S

Published Date

  • July 1, 2014

Published In

Volume / Issue

  • 86 / 13

Start / End Page

  • 6683 - 6688

PubMed ID

  • 24856003

Pubmed Central ID

  • PMC4362721

Electronic International Standard Serial Number (EISSN)

  • 1520-6882

Digital Object Identifier (DOI)

  • 10.1021/ac501436d

Language

  • eng

Conference Location

  • United States