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Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons.

Publication ,  Journal Article
Kalmbach, BE; Hodge, RD; Jorstad, NL; Owen, S; de Frates, R; Yanny, AM; Dalley, R; Mallory, M; Graybuck, LT; Radaelli, C; Keene, CD; Gwinn, RP ...
Published in: Neuron
September 15, 2021

In the neocortex, subcerebral axonal projections originate largely from layer 5 (L5) extratelencephalic-projecting (ET) neurons. The unique morpho-electric properties of these neurons have been mainly described in rodents, where retrograde tracers or transgenic lines can label them. Similar labeling strategies are infeasible in the human neocortex, rendering the translational relevance of findings in rodents unclear. We leveraged the recent discovery of a transcriptomically defined L5 ET neuron type to study the properties of human L5 ET neurons in neocortical brain slices derived from neurosurgeries. Patch-seq recordings, where transcriptome, physiology, and morphology were assayed from the same cell, revealed many conserved morpho-electric properties of human and rodent L5 ET neurons. Divergent properties were often subtler than differences between L5 cell types within these two species. These data suggest a conserved function of L5 ET neurons in the neocortical hierarchy but also highlight phenotypic divergence possibly related to functional specialization of human neocortex.

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Published In

Neuron

DOI

EISSN

1097-4199

Publication Date

September 15, 2021

Volume

109

Issue

18

Start / End Page

2914 / 2927.e5

Location

United States

Related Subject Headings

  • Transcriptome
  • Pyramidal Cells
  • Patch-Clamp Techniques
  • Organ Culture Techniques
  • Neurology & Neurosurgery
  • Neocortex
  • Morphogenesis
  • Middle Aged
  • Mice, Transgenic
  • Mice, Inbred C57BL
 

Citation

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Kalmbach, B. E., Hodge, R. D., Jorstad, N. L., Owen, S., de Frates, R., Yanny, A. M., … Ting, J. T. (2021). Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons. Neuron, 109(18), 2914-2927.e5. https://doi.org/10.1016/j.neuron.2021.08.030
Kalmbach, Brian E., Rebecca D. Hodge, Nikolas L. Jorstad, Scott Owen, Rebecca de Frates, Anna Marie Yanny, Rachel Dalley, et al. “Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons.Neuron 109, no. 18 (September 15, 2021): 2914-2927.e5. https://doi.org/10.1016/j.neuron.2021.08.030.
Kalmbach BE, Hodge RD, Jorstad NL, Owen S, de Frates R, Yanny AM, et al. Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons. Neuron. 2021 Sep 15;109(18):2914-2927.e5.
Kalmbach, Brian E., et al. “Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons.Neuron, vol. 109, no. 18, Sept. 2021, pp. 2914-2927.e5. Pubmed, doi:10.1016/j.neuron.2021.08.030.
Kalmbach BE, Hodge RD, Jorstad NL, Owen S, de Frates R, Yanny AM, Dalley R, Mallory M, Graybuck LT, Radaelli C, Keene CD, Gwinn RP, Silbergeld DL, Cobbs C, Ojemann JG, Ko AL, Patel AP, Ellenbogen RG, Bakken TE, Daigle TL, Dee N, Lee BR, McGraw M, Nicovich PR, Smith K, Sorensen SA, Tasic B, Zeng H, Koch C, Lein ES, Ting JT. Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons. Neuron. 2021 Sep 15;109(18):2914-2927.e5.
Journal cover image

Published In

Neuron

DOI

EISSN

1097-4199

Publication Date

September 15, 2021

Volume

109

Issue

18

Start / End Page

2914 / 2927.e5

Location

United States

Related Subject Headings

  • Transcriptome
  • Pyramidal Cells
  • Patch-Clamp Techniques
  • Organ Culture Techniques
  • Neurology & Neurosurgery
  • Neocortex
  • Morphogenesis
  • Middle Aged
  • Mice, Transgenic
  • Mice, Inbred C57BL