Signature morpho-electric, transcriptomic, and dendritic properties of human layer 5 neocortical pyramidal neurons.

Journal Article (Journal Article)

In the neocortex, subcerebral axonal projections originate largely from layer 5 (L5) extratelencephalic-projecting (ET) neurons. The unique morpho-electric properties of these neurons have been mainly described in rodents, where retrograde tracers or transgenic lines can label them. Similar labeling strategies are infeasible in the human neocortex, rendering the translational relevance of findings in rodents unclear. We leveraged the recent discovery of a transcriptomically defined L5 ET neuron type to study the properties of human L5 ET neurons in neocortical brain slices derived from neurosurgeries. Patch-seq recordings, where transcriptome, physiology, and morphology were assayed from the same cell, revealed many conserved morpho-electric properties of human and rodent L5 ET neurons. Divergent properties were often subtler than differences between L5 cell types within these two species. These data suggest a conserved function of L5 ET neurons in the neocortical hierarchy but also highlight phenotypic divergence possibly related to functional specialization of human neocortex.

Full Text

Duke Authors

Cited Authors

  • Kalmbach, BE; Hodge, RD; Jorstad, NL; Owen, S; de Frates, R; Yanny, AM; Dalley, R; Mallory, M; Graybuck, LT; Radaelli, C; Keene, CD; Gwinn, RP; Silbergeld, DL; Cobbs, C; Ojemann, JG; Ko, AL; Patel, AP; Ellenbogen, RG; Bakken, TE; Daigle, TL; Dee, N; Lee, BR; McGraw, M; Nicovich, PR; Smith, K; Sorensen, SA; Tasic, B; Zeng, H; Koch, C; Lein, ES; Ting, JT

Published Date

  • September 15, 2021

Published In

Volume / Issue

  • 109 / 18

Start / End Page

  • 2914 - 2927.e5

PubMed ID

  • 34534454

Pubmed Central ID

  • PMC8570452

Electronic International Standard Serial Number (EISSN)

  • 1097-4199

Digital Object Identifier (DOI)

  • 10.1016/j.neuron.2021.08.030


  • eng

Conference Location

  • United States