Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer.

Journal Article (Journal Article)

The diverse malignant, stromal, and immune cells in tumors affect growth, metastasis, and response to therapy. We profiled transcriptomes of ∼6,000 single cells from 18 head and neck squamous cell carcinoma (HNSCC) patients, including five matched pairs of primary tumors and lymph node metastases. Stromal and immune cells had consistent expression programs across patients. Conversely, malignant cells varied within and between tumors in their expression of signatures related to cell cycle, stress, hypoxia, epithelial differentiation, and partial epithelial-to-mesenchymal transition (p-EMT). Cells expressing the p-EMT program spatially localized to the leading edge of primary tumors. By integrating single-cell transcriptomes with bulk expression profiles for hundreds of tumors, we refined HNSCC subtypes by their malignant and stromal composition and established p-EMT as an independent predictor of nodal metastasis, grade, and adverse pathologic features. Our results provide insight into the HNSCC ecosystem and define stromal interactions and a p-EMT program associated with metastasis.

Full Text

Duke Authors

Cited Authors

  • Puram, SV; Tirosh, I; Parikh, AS; Patel, AP; Yizhak, K; Gillespie, S; Rodman, C; Luo, CL; Mroz, EA; Emerick, KS; Deschler, DG; Varvares, MA; Mylvaganam, R; Rozenblatt-Rosen, O; Rocco, JW; Faquin, WC; Lin, DT; Regev, A; Bernstein, BE

Published Date

  • December 14, 2017

Published In

Volume / Issue

  • 171 / 7

Start / End Page

  • 1611 - 1624.e24

PubMed ID

  • 29198524

Pubmed Central ID

  • PMC5878932

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2017.10.044


  • eng

Conference Location

  • United States