Sulfated glycopeptide nanostructures for multipotent protein activation.

Journal Article (Journal Article)

Biological systems have evolved to utilize numerous proteins with capacity to bind polysaccharides for the purpose of optimizing their function. A well-known subset of these proteins with binding domains for the highly diverse sulfated polysaccharides are important growth factors involved in biological development and tissue repair. We report here on supramolecular sulfated glycopeptide nanostructures, which display a trisulfated monosaccharide on their surfaces and bind five critical proteins with different polysaccharide-binding domains. Binding does not disrupt the filamentous shape of the nanostructures or their internal β-sheet backbone, but must involve accessible adaptive configurations to interact with such different proteins. The glycopeptide nanostructures amplified signalling of bone morphogenetic protein 2 significantly more than the natural sulfated polysaccharide heparin, and promoted regeneration of bone in the spine with a protein dose that is 100-fold lower than that required in the animal model. These highly bioactive nanostructures may enable many therapies in the future involving proteins.

Full Text

Duke Authors

Cited Authors

  • Lee, SS; Fyrner, T; Chen, F; Álvarez, Z; Sleep, E; Chun, DS; Weiner, JA; Cook, RW; Freshman, RD; Schallmo, MS; Katchko, KM; Schneider, AD; Smith, JT; Yun, C; Singh, G; Hashmi, SZ; McClendon, MT; Yu, Z; Stock, SR; Hsu, WK; Hsu, EL; Stupp, SI

Published Date

  • August 2017

Published In

Volume / Issue

  • 12 / 8

Start / End Page

  • 821 - 829

PubMed ID

  • 28650443

Pubmed Central ID

  • PMC5553550

Electronic International Standard Serial Number (EISSN)

  • 1748-3395

Digital Object Identifier (DOI)

  • 10.1038/nnano.2017.109


  • eng

Conference Location

  • England