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Transcription factor AP-2 regulates human immunodeficiency virus type 1 gene expression.

Publication ,  Journal Article
Perkins, ND; Agranoff, AB; Duckett, CS; Nabel, GJ
Published in: J Virol
October 1994

Human immunodeficiency virus type 1 (HIV-1) gene expression is regulated by an enhancer region composed of multiple potential cis-acting regulatory sites. Here, we describe binding sites for the transcription factor AP-2 in the HIV-1 long terminal repeat which modulate HIV enhancer function. One site is embedded within the two previously described kappa B elements, and a second site is detected further downstream. DNase I footprinting and electrophoretic mobility shift assay experiments demonstrated that AP-2 binds to the site between the kappa B elements. Interestingly, AP-2 and NF-kappa B bind to this region in a mutually exclusive manner. Mutations which disrupt this AP-2-binding site lower basal levels of transcription but do not affect NF-kappa B-mediated induction by tumor necrosis factor alpha in Jurkat T leukemia cells.

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Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

October 1994

Volume

68

Issue

10

Start / End Page

6820 / 6823

Location

United States

Related Subject Headings

  • Virology
  • Transcription Factors
  • Transcription Factor AP-2
  • TATA Box
  • Regulatory Sequences, Nucleic Acid
  • Recombinant Proteins
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Humans
  • HIV-1
 

Citation

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Perkins, N. D., Agranoff, A. B., Duckett, C. S., & Nabel, G. J. (1994). Transcription factor AP-2 regulates human immunodeficiency virus type 1 gene expression. J Virol, 68(10), 6820–6823. https://doi.org/10.1128/JVI.68.10.6820-6823.1994
Perkins, N. D., A. B. Agranoff, C. S. Duckett, and G. J. Nabel. “Transcription factor AP-2 regulates human immunodeficiency virus type 1 gene expression.J Virol 68, no. 10 (October 1994): 6820–23. https://doi.org/10.1128/JVI.68.10.6820-6823.1994.
Perkins ND, Agranoff AB, Duckett CS, Nabel GJ. Transcription factor AP-2 regulates human immunodeficiency virus type 1 gene expression. J Virol. 1994 Oct;68(10):6820–3.
Perkins, N. D., et al. “Transcription factor AP-2 regulates human immunodeficiency virus type 1 gene expression.J Virol, vol. 68, no. 10, Oct. 1994, pp. 6820–23. Pubmed, doi:10.1128/JVI.68.10.6820-6823.1994.
Perkins ND, Agranoff AB, Duckett CS, Nabel GJ. Transcription factor AP-2 regulates human immunodeficiency virus type 1 gene expression. J Virol. 1994 Oct;68(10):6820–6823.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

October 1994

Volume

68

Issue

10

Start / End Page

6820 / 6823

Location

United States

Related Subject Headings

  • Virology
  • Transcription Factors
  • Transcription Factor AP-2
  • TATA Box
  • Regulatory Sequences, Nucleic Acid
  • Recombinant Proteins
  • Promoter Regions, Genetic
  • Molecular Sequence Data
  • Humans
  • HIV-1